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RE-ORGA, a Korean Herb Extract, Can Prevent Hair Loss Induced by Dihydrotestosterone in Human Dermal Papilla Cells

Annals of Dermatology 2019년 31권 5호 p.530 ~ 537
 ( Kang Myung-Gyun ) - Korea Institute of Toxicology Department of Predictive Toxicology

 ( Park Dae-Ui ) - Korea Institute of Toxicology Department of Predictive Toxicology
 ( Han Hyoung-Yun ) - Korea Institute of Toxicology Department of Predictive Toxicology
 ( Shim Hye-eun ) - Wooridul Huebrain Ltd. Research Institute
 ( Hong Yoon-jung ) - Wooridul Huebrain Ltd. Research Institute
 ( Moon Ji-Yeon ) - Winnova Co., Ltd Research Institute
 ( Yoon Seok-Joo ) - Korea Institute of Toxicology Department of Predictive Toxicology
 ( Kwon Bo-sun ) - Wooridul Huebrain Ltd. Research Institute

Abstract


Background: Androgenic alopecia (AGA) is the most common type of hair loss. It is likely inherited genetically and is promoted by dihydrotestosterone. 5α-reductase has been proven a good target through finasteride use. However, the pathogenesis of AGA cannot be fully explained based only on dihydrotestosterone levels.

Objective: To identify similar hairloss inhibition activity of RE-ORGA with mode of action other than finasteride.

Methods: We prepared RE-ORGA from Korean herb mixtures. We performed MTT assays for cytotoxicity, Cell Counting Kit-8 assays for cell proliferation, and western blot to identify expression levels of 5α-reductase and Bax. RNA-sequencing was performed for the expression patterns of genes in dihydrotestosterone-activated pathways. Anti-inflammatory activity was also assessed by the expression levels of tumor necrosis factor-alpha (TNF-α) and interleukin 6.

Results: REORGA could promote the proliferation of human dermal papilla cells and showed low cytotoxicity. It also inhibited the expression of 5α-reductases and Bax in the cells. RNA-sequencing results verified that the mRNA expressions of SRD5A1, Bax, transforming growth factor-beta 1 (TGF-β1), and TGF-β1 induced transcript 1 (TGFβ1I1) were decreased, whereas expression of protein tyrosine kinase 2 beta (PTK2β) was more elevated. REORGA also showed anti-inflammatory activity through decreased mRNA levels of TNF-α.

Conclusion: Transcriptionally, up-regulation of PTK2β and concomitant down-regulation of TGFβ1I1 imply that RE-ORGA can modulate androgen receptor sensitivity, decreasing the expression of 5α-reductase type II and Bax together with TGF-β1 transcripts; RE-ORGA also showed partial anti-inflammatory activity. Overall, RE-ORGA is expected to alleviate hair loss by regulating 5α-reductase activity and the receptor's androgen sensitivity.

키워드

Androgenic alopecia; Dihydrotestosterone; Finasteride; Herbal extract; 5 alpha-reductase
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