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The Histochemical Changes of Cholinesterase Activity in Rabbit´s Retinae at Early Postnatal Stages

Yonsei Medical Journal 1964년 5권 1호 p.33 ~ 36
 ( Park Soo-Yun ) - 연세대학교 의과대학 해부학교실

 ( Choi Dum-Duck ) - 연세대학교 의과대학 해부학교실

Abstract


The authors studied histochemically the enzymatic sites of retinal cholinesterase activity in the albino rabbit during different early postnatal periods. The retinae of young rabbits weighing approximately 60 gm. whose palpebrae were still fused and unable to function for sight, and of rabbits weighing approximately 300 gm. which were able to see, were obtained immediately after killing the animals by the intravenous injection of air or by decapitation and fixed in formalin-sucrose ammonia fixative, as recommended by Pearson (1963), for about 24 hours and incubated in the substrate containing acetylthiocholine, iodide, as presented by Gerebtzoff (1953), at 37degrees C. for 2 to 3 hours. After the treatment of the retinae, as devised by Koelle and Friedenwald (1950), the retinae were sectioned at 5 micra and mounted both without counterstaining and after counterstaining with hematoxylin alone.

In young rabbits weighing approximately 60 gm., moderate cholinesterase activity was observed only in the ganglion cells of the retinae and slight enzymatic activity was faintly apparent in the layer of the optic nerve fibers. No enzymatic activity was recognized in the remaining layers of the retinae.

In rabbits weighing approximately 300 gm. the retinae showed different enzymatic distribution compared to the former. The cholinesterase activity was diversely distributed compared to the former and was localized in the inner nuclear layer, inner plexiform layer, and the ganglion cells showing moderate to slight activity.

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