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An Autoradiographic Study on Metabolism of Serotonin in Cerebral Nerve Cells Using 5-Hydroxytryptophan-C14

Yonsei Medical Journal 1967년 8권 1호 p.8 ~ 12
 ( Choi Kum-Duck ) - 연세대학교 의과대학 해부학교실

 ( Ahn Seung-Bong ) - 연세대학교 의과대학
오영근 ( Oh Yung-Keun ) - 연세대학교 의과대학 해부학교실
 ( Rhim Kyu-Soon ) - 연세대학교 의과대학 해부학교실
 ( Moon Byong-Yull ) - 연세대학교 의과대학

Abstract


In order to demonstrate autoradiographically the sites of serotonin metabolism in the brain, DL 5-HTP and DL 5-HTP-C14 were intraperitoneally administered to healthy adult mice. In order to distinguish histochemically serotonin-like substances which have staining characteristics similar to the enterochromaffin cells of the gastrointestinal tract, serotonin-releaser reserpine was administered intravenously to healthy adult rabbits. A stripping film technique for autoradiography, a ferric ferricyanide reduction test by Schmorl, and a Gomori-Burtner methenamine silver staining method for argentaffin cells were used in this study. In the brain tissues of mice treated with 5-HTP, it was observed that the cytoplasm of the nerve cells, of the cerebral cortex had blue positive staining substances by the ferric ferricyanide technique. In similar tissue sites in mice treated with 5-HTP-C14, a number of blackened-par-ticles reduced by beta rays were easily found. especially in the cytoplasm of nerve cells and neuroglia cells. It is suggested that the serotonin precursor, DL 5-hydrox-ytryptophan is metabolized the cerebral tissue, and serotonin is synthesized also in the nerve cells and the neuroglia cells.

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