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Urea cycle 中間代謝物質(L-arginine 및 DL-ornithine)의 投與가 白鼠摘出 肝臟의 Ammonia 解毒機能에 미치는 影響

The Effect of Urea Cycle Intermediates(L-arginine 및 DL-ornithine) on the Ammonia Detoxication in Perfused Rat Liver

경북의대잡지 1967년 8권 2호 p.305 ~ 312
황일우 (  ) - 경북대학교 의과대학 생화학교실

Abstract


The ability of urea cycle intermediates (Larginine, DL-ornithine) to decrease the blood ammonia was investigated in perfused rat liver.
The decrease of exogenously added ammonia (ammonia-~´, 500 and 1000,ug/100m1) in blood perfusate was not affected by addition of the urea cycle intermediates (100nmg,/100m1) in the first 2 or 3 hours. FoIlo~ving this period, in the control group perfused with the blood perfusa*_e containing normal rat plasma amino acid concentration, the decrease was significantly weakened; whereas in arginine or ornithine added group, it showed a steady decrease throughout 5 hours perfusion.
In control group, the arginine concentration in perfusate decreased continously during perfusion to reach about 60 0 of the initial concentration after 5 hours. On the other hand, in arginine added group, the concentration maintained about5 times the nor:ral rat plasma concentration, even though the great part of the arginine was broken by liver arginase.
Urea concentration in the perfusate showed a great increase in the first one hour of perfusion.: when arginine was added, but thereafter the increase of urea was almost equal to that in the control group. Vo difference in urea production between DL-ornithine added group and control` group was noted.
Glucose consumption and ceLuIar transport of a-amirioisobut}-ric acid-C´ ~ were also determined without any noticeable difference each etiperiment.
Above result suggest that normal ammonia_ detotiication function in liver may net be accerelated by administration of arginine or ornithine, but when the concentration of these intermediates. in bloo{ decrease, the fu.ZCtion tends to decrease.

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