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Streptomycin耐性 Mycobacterium phlei에서 抽出한 DNA에 依한 Streptomycin耐性에 對하여

Transformation of Streptomycin Resistance in Mycobacterium phlei by DNA from Streptomycin-resistance Strain

경북의대잡지 1970년 11권 1호 p.99 ~ 107
김재식 (  ) - 慶北大學校 醫科大學 臨床病理學敎室

金重明 (  ) - 慶北大學校 醫科大學 臨床病理學敎室

Abstract


Experimental studies on the transformation of streptomycin (S`.4) resistance in Jlycobacterium (M) phiei were carried cut with DNAR derived from S=Vi-resistant culture.
Two methods were applied for t´-.e study; one was culture method in which the bacterial cells to be trans= ormed were culture/ with DNAR, and the other was resting cell method in which the cells were treated with the DN AR at C C and 37C.
After the treatment, cells were cultured on media containing Si~1 in serial concentrations to determine the increase in the resistance. The results were as follows.
1. The SM resistance of DNAR-treated cells ; by culture method was markedly increased up to 1, 000 - 5, G00 mcg/ml as compared with the original cells (i,, mcg; :nl) .
2. No significant SI~I resistance was observed by the resting cell method treated at 0C.
3. The :esistance to Sli increased up to 5, C~0 mcg/r_21 in. cells washed after treatment with DNAR at 37C ands subcultured en original media. I´_owever, unwashed cells did not show any significant increase in the resistance.
4. It was conceivable with the results obtained that SM resistance can be transformed in . M. phlei.

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