medric medric
[닫기]
잠시만 기다려 주세요. 로딩중입니다.

Simultaneous determination and difference evaluation of 14 ginsenosides in Panax ginseng roots cultivated in different areas and ages by high-performance liquid chromatography coupled with triple quadrupole mass spectrometer in the multiple reaction?monitoring mode combined with multivariate statistical analysis

Journal of Ginseng Research 2019년 43권 4호 p.508 ~ 516
 ( Xiu Yang ) - Chang Chun University of Chinese Medicine Jilin Ginseng Academy

 ( Li Xue ) - Chang Chun University of Chinese Medicine Jilin Ginseng Academy
 ( Sun Xiuli ) - Chang Chun University of Chinese Medicine Jilin Ginseng Academy
 ( Xiao Dan ) - Chang Chun University of Technology
 ( Miao Rui ) - Chang Chun University of Chinese Medicine Jilin Ginseng Academy
 ( Zhao Huanxi ) - Chang Chun University of Chinese Medicine Jilin Ginseng Academy
 ( Liu Shuying ) - Chang Chun University of Chinese Medicine Jilin Ginseng Academy

Abstract


Background: Ginsenosides are not only the principal bioactive components but also the important indexes to the quality assessment of Panax ginseng Meyer. Their contents in cultivated ginseng vary with the growth environment and age. The present study aimed at evaluating the significant difference between 36 cultivated ginseng of different cultivation areas and ages based on the simultaneously determined contents of 14 ginsenosides.

Methods: A high-performance liquid chromatography (HPLC) coupled with triple quadrupole mass spectrometer (MS) method was developed and used in the multiple reaction?monitoring (MRM) mode (HPLC-MRM/MS) for the quantitative analysis of ginsenosides. Multivariate statistical analysis, such as principal component analysis and partial least squares-discriminant analysis, was applied to discriminate ginseng samples of various cultivation areas and ages and to discover the differentially accumulated ginsenoside markers.

Results: The developed HPLC-MRM/MS method was validated to be precise, accurate, stable, sensitive, and repeatable for the simultaneous determination of 14 ginsenosides. It was found that the 3- and 5-yr-old ginseng samples were differentiated distinctly by all means of multivariate statistical analysis, whereas the 4-yr-old samples exhibited similarity to either 3- or 5-yr-old samples in the contents of ginsenosides. Among the 14 detected ginsenosides, Rg1, Rb1, Rb2, Rc, 20(S)-Rf, 20(S)-Rh1, and Rb3 were identified as potential markers for the differentiation of cultivation ages. In addition, the 5-yr-old samples were able to be classified in cultivation area based on the contents of ginsenosides, whereas the 3- and 4-yr-old samples showed little differences in cultivation area.

Conclusion: This study demonstrated that the HPLC-MRM/MS method combined with multivariate statistical analysis provides deep insight into the accumulation characteristics of ginsenosides and could be used to differentiate ginseng that are cultivated in different areas and ages.

키워드

Cultivation area and age; Ginsenoside; High-performance liquid chromatography coupled with triple quadrupole mass spectrometer in the multiple reaction?monitoring mode; Multivariate statistical analysis; Panax ginseng
원문 및 링크아웃 정보
 
등재저널 정보
KCI