medric medric
[닫기]
잠시만 기다려 주세요. 로딩중입니다.

Ginseng extracts modulate mitochondrial bioenergetics of live cardiomyoblasts: a functional comparison of different extraction solvents

Journal of Ginseng Research 2019년 43권 4호 p.517 ~ 526
 ( Huang Yun ) - Hong Kong University of Science and Technology Division of Life Science

 ( Kwan Kenneth Kin Leung ) - Hong Kong University of Science and Technology Division of Life Science
 ( Leung Ka Wing ) - Hong Kong University of Science and Technology Division of Life Science
 ( Yao Ping ) - Hong Kong University of Science and Technology Division of Life Science
 ( Wang Huaiyou ) - Hong Kong University of Science and Technology Division of Life Science
 ( Dong Tina Tingxia ) - Hong Kong University of Science and Technology Division of Life Science
 ( Tsim Karl Wah Keung ) - Hong Kong University of Science and Technology Division of Life Science

Abstract


Background: The root of Panax ginseng, a member of Araliaceae family, has been used as herbal medicine and functional food in Asia for thousands of years. According to Traditional Chinese medicine, ginseng is the most widely used “Qi-invigorating” herbs, which provides tonic and preventive effects by resisting oxidative stress, influencing energy metabolism, and improving mitochondrial function. Very few reports have systematically measured cell mitochondrial bioenergetics after ginseng treatment.

Methods: Here, H9C2 cell line, a rat cardiomyoblast, was treated with ginseng extracts having extracted using solvents of different polarity, i.e., water, 50% ethanol, and 90% ethanol, and subsequently, the oxygen consumption rate in healthy and tert-butyl hydroperoxide?treated live cultures was determined by Seahorse extracellular flux analyzer.

Results: The 90% ethanol extracts of ginseng possessed the strongest antioxidative and tonic activities to mitochondrial respiration and therefore provided the best protective effects to H9C2 cardiomyocytes. By increasing the spare respiratory capacity of stressed H9C2 cells up to three-folds of that of healthy cells, the 90% ethanol extracts of ginseng greatly improved the tolerance of myocardial cells to oxidative damage.

Conclusion: These results demonstrated that the low polarity extracts of ginseng could be the best extract, as compared with others, in regulating the oxygen consumption rate of cultured cardiomyocytes during mitochondrial respiration.

키워드

Cell protection; Extracellular flux analyzer; Ginseng Radix; Live cell; Reactive oxygen species
원문 및 링크아웃 정보
 
등재저널 정보
KCI