medric medric
[닫기]
잠시만 기다려 주세요. 로딩중입니다.

Comparative transcriptome analysis of heat stress responsiveness between two contrasting ginseng cultivars

Journal of Ginseng Research 2019년 43권 4호 p.572 ~ 579
 ( Jayakodi Murukarthick ) - Seoul National University College of Agriculture and Life Sciences Department of Plant Science

 ( Lee Sang-Choon ) - Seoul National University College of Agriculture and Life Sciences Department of Plant Science
 ( Yang Tae-Jin ) - Seoul National University College of Agriculture and Life Sciences Department of Plant Science

Abstract


Background: Panax ginseng has been used in traditional medicine to strengthen the body and mental well-being of humans for thousands of years. Many elite ginseng cultivars have been developed, and ginseng cultivation has become well established during the last century. However, heat stress poses an important threat to the growth and sustainable production of ginseng. Efforts have been made to study the effects of high temperature on ginseng physiology, but knowledge of the molecular responses to heat stress is still limited.

Methods: We sequenced the transcriptomes (RNA-Seq) of two ginseng cultivars, Chunpoong (CP) and Yunpoong (YP), which are sensitive and resistant to heat stress, respectively, after 1- and 3-week heat treatments. Differential gene expression and gene ontology enrichment along with profiled chlorophyll contents were performed.

Results: CP is more sensitive to heat stress than YP and exhibited a lower chlorophyll content than YP. Moreover, heat stress reduced the chlorophyll content more rapidly in CP than in YP. A total of 329 heat-responsive genes were identified. Intriguingly, genes encoding chlorophyll a/b?binding proteins, WRKY transcription factors, and fatty acid desaturase were predominantly responsive during heat stress and appeared to regulate photosynthesis. In addition, a genome-wide scan of photosynthetic and sugar metabolic genes revealed reduced transcription levels for ribulose 1,5-bisphosphate carboxylase/oxygenase under heat stress, especially in CP, possibly attributable to elevated levels of soluble sugars.

Conclusion: Our comprehensive genomic analysis reveals candidate loci/gene targets for breeding and functional studies related to developing high temperature?tolerant ginseng varieties.

키워드

Chunpoong; Heat stress; Panax ginseng; RNA-Seq; Yunpoong
원문 및 링크아웃 정보
 
등재저널 정보
KCI