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Two new triterpenoid saponins derived from the leaves of Panax ginseng and their antiinflammatory activity

Journal of Ginseng Research 2019년 43권 4호 p.600 ~ 605
 ( Li Fu ) - Chinese Academy of Sciences Chengdu Institute of Biology

 ( Cao Yufeng ) - Huaiyin Normal University Jiangsu Collaborative Innovation Center of Regional Modern Agriculture & Environmental Protection
 ( Luo Yanyan ) - Huaiyin Normal University Jiangsu Collaborative Innovation Center of Regional Modern Agriculture & Environmental Protection
 ( Liu Tingwu ) - Huaiyin Normal University Jiangsu Collaborative Innovation Center of Regional Modern Agriculture & Environmental Protection
 ( Yan Guilong ) - Huaiyin Normal University Jiangsu Collaborative Innovation Center of Regional Modern Agriculture & Environmental Protection
 ( Chen Liang ) - Huaiyin Normal University Jiangsu Collaborative Innovation Center of Regional Modern Agriculture & Environmental Protection
 ( Ji Lilian ) - Huaiyin Normal University Jiangsu Collaborative Innovation Center of Regional Modern Agriculture & Environmental Protection
 ( Wang Lun ) - Chinese Academy of Sciences Chengdu Institute of Biology
 ( Chen Bin ) - Chinese Academy of Sciences Chengdu Institute of Biology
 ( Yaseen Aftab ) - Chinese Academy of Sciences Chengdu Institute of Biology
 ( Khan Ashfaq A. ) - Women University of Azad Jammu and Kashmir Department of Chemistry
 ( Zhang Guolin ) - Chinese Academy of Sciences Chengdu Institute of Biology
 ( Jiang Yunyao ) - China Academy of Chinese Medical Sciences Xiyuan Hospital
 ( Liu Jianxun ) - China Academy of Chinese Medical Sciences Xiyuan Hospital
 ( Wang Gongcheng ) - Nanjing Medical University Huaian No. 1 People’s Hospital Department of Urology
 ( Wang Ming-Kui ) - Chinese Academy of Sciences Chengdu Institute of Biology
 ( Hu Weicheng ) - Huaiyin Normal University Jiangsu Collaborative Innovation Center of Regional Modern Agriculture & Environmental Protection

Abstract


Background: The leaves and roots of Panax ginseng are rich in ginsenosides. However, the chemical compositions of the leaves and roots of P. ginseng differ, resulting in different medicinal functions. In recent years, the aerial parts of members of the Panax genus have received great attention from natural product chemists as producers of bioactive ginsenosides. The aim of this study was the isolation and structural elucidation of novel, minor ginsenosides in the leaves of P. ginseng and evaluation of their antiinflammatory activity in vitro.

Methods: Various chromatographic techniques were applied to obtain pure individual compounds, and their structures were determined by nuclear magnetic resonance and high-resolution mass spectrometry, as well as chemical methods. The antiinflammatory effect of the new compounds was evaluated on lipopolysaccharide-stimulated RAW 264.7 cells.

Results and conclusions: Two novel, minor triterpenoid saponins, ginsenoside LS1 (1) and 5,6-didehydroginsenoside Rg3 (2), were isolated from the leaves of P. ginseng. The isolated compounds 1 and 2 were assayed for their inhibitory effect on nitric oxide production in LPS-stimulated RAW 264.7 cells, and Compound 2 showed a significant inhibitory effect with IC50 of 37.38 μM compared with that of NG-monomethyl-L-arginine (IC50 = 90.76 μM). Moreover, Compound 2 significantly decreased secretion of cytokines such as prostaglandin E2 and tumor necrosis factor-α. In addition, Compound 2 significantly suppressed protein expression of inducible nitric oxide synthase and cyclooxygenase-2. These results suggested that Compound 2 could be used as a valuable candidate for medicinal use or functional food, and the mechanism is warranted for further exploration.

키워드

Antiinflammatory activity; Panax ginseng; Triterpenoid saponins
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