Serotonin성 약제가 쥐의 하복신경 자극 후 정관내압에 미치는 영향
Effects of Serotonergic Drugs on Intraluminal Pressure of Vas Deferent Induced by Electrical Stimulation of Rat Hypogastric Nerve
서경근 ( Seo Kyung-Keun ) - 중앙대학교 의과대학 비뇨기과학교실
곽승민 ( Kwak Seung-Min ) - 중앙대학교 의과대학 비뇨기과학교실
김세철 ( Kim Sae-Chul ) - 중앙대학교 의과대학 비뇨기과학교실
Abstract
결론
여러 가지 serotonin성 약제가 사정을 지연시키는 작용기전의 일단을 규명하고자 이들 약
제를 정맥내 투여 전·후에 하복신경을 전기자극하여 정관내압의 변화를 생리식염수 투여군
과 비교관찰하였다.
1. 반복적인 전기자극으로 인한 하복신경의 손상, 장시간 (3시간 이상) 마취, 반복적인 생
리식염수 투여, DMSO등은 정관내압의 변화를 초래하지 않았다.
2. clomipramine, fluoxetine, sertraline, paroxetine이 하복신경의 전기자극에 의한 정관내
압상승을 억제하는 효과를 비교하였을 때, clomipramine에 의한 억제효과가 가장 좋았으며,
다음은 sertraline과 paroxetine이었고 fluoxetine의 억제효과가 가장 낮았으며 (p<0.05,
ANOVA), sertraline과 paroxetine 투여 군간에는 유의한 효과 차이가 없었다.
3. 각 약제의 치료 용량의 20배 농도를 투여한 군에서 억제효과가 없었던 동물은
clomipramine은 1례도 없었으며, sertaline 1례 (20%), paroxetine 2례 (40%), fluoxetine 4례
(80%)였다.
4. clomipramine의 말초 작용에 의한 정관내압상승의 억제효과는 10배 용량에서 58.96±
6.39%로 중추작용효과(66.74±8.97%)와 유의한 차이가 없었으나 10배 용량의 sertraline 투
여군은 60%에서 말초효과가 관찰되지 않았다.
결론적으로 clomipramine은 SSRI보다 정관내압에 대한 억제효과가 강하며, 이것은
clornipramine의 보다 강력한 말초 작용에 기인할 지도 모른다.
Purpose: To compare the effects of various serotonergic drugs on the inhibition of
intraluminal pressure rise in the rat vas deferens induced by electrical stimulation of the
hypogastric nerve.
Material and Methods: Twenty-five Sprague Dawley rats (250∼300 gm) were
randomly divided into five groups of five animals each, which received intravenous
injection of normal saline, clomipramine, sertraline, paroxetine, or fluoxetine. Before
(baseline pressure) and 30 minutes after intravenous injection of four different doses (0.1
to 20 the therapeutic dose) of each agent, the hypogastric nerve, identified using
microsurgical technique, was electrically stimulated, and the intraluminal pressure of the
vats deferens was measured (central effect group). To evaluate the peripheral effects of
clomipramine and sertraline, intraluminal vassal pressure was also measured after
transaction of all proximal sympathetic nerves projecting to the hypogastric nerve and
the commissural branches between the right and left major and accessory pelvic ganglia.
The adrenal veins were ligated bilaterally.
Results: Repeated stimulation of the hypogastric nerves, anesthesia of long duration (3
hours), and repeated intravenous injection of normal saline did not result in significant
changes in the intraluminal pressure of the vats deferens. All serotonergic agents
inhibited elevation of the intraluminal pressure of the vas deferens in a dosedependent
manner (p<0.05). The extent of inhibition by 20-fold therapeutic doses of clomipramine,
sertraline, paroxetine and fluoxetine were 74.4±1.8%, 34.1±8.3%, 24.8±7.8%, and 8.1±
3.5%, respectively. At doses 10- and 20-fold the therapeutic dose, clomipramine had the
strongest inhibitory effect, followed by sertraline and paroxetine, then fluoxetine
(p<0.05). Definite inhibition was noted in all rats receiving clomipramine at 10- and
20-fold the therapeutic dose; the degree of inhibition was 80% in the sertraline-, 60% in
the paroxetine-, and 20% in the fluoxetine-treated group. The inhibitory effect of
sertraline on the elevation of the intraluminal vasal pressure in the peripheral-effect
group was significantly (p<0.01) less than that in the central-effect group. However,
there was no difference in the inhibitory effect of clomipramine in the two groups.
Conclusion: Clomipramine was the most potent inhibitor of the elevation of the
intraluminal pressure of the rat vats after electrical stimulation of the hypogastric nerve.
The greater effect might be attributable to an additional peripheral effect of this drug on
the was deferens.
키워드
Vas deferens; Electrical stimulation; Hypogastric nerve; Rat; Serotonergic agent;
KMID :
0356419990170020099
DOI :
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