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昆蟲組織培養細胞(Antheraea eucalypti Ovarian Cells)에 있어서 日本腦炎바이러스 增殖

Growth of Japanese Encephalitis Virus in Insect(Grace´s Antheraea Cell Line) Tissue Culture

대한미생물학회지 1969년 4권 1호 p.13 ~ 20
李淵台 (  ) - 가톨릭大學 醫學部 微生物學敎室

蘇特 (  ) - 慶北市美國海軍第二醫學硏究所 節足動物組織培養室

Abstract


Grace´s line of Antheraea eucalypti ovarian cell has been adapted by Suitor to a medium consisting of Grace´s insect cell culture medium(GMA) plus some additives.
A homogenate of a Japan strain of JEV(JaGar) isolated from mosquito and Korean strain of JEV (HM81) isolated from suckling mice, both with mice brains were used as inoculums. Virus-cell culture was carried out at 28°C and fresh hemolymph in GMA was added usually after 7, 14 and 21st, days in order to maintain the cells over period of 5 weeks for completion of the tests. Virus-cell mixture were prepared as serial ten-fold dilutions in phosphate buffered saline and 0.5% bovine albumin, 0.03 ml of virus emulsion was inoculated intracerebrally into weaning mice to determine LD50.
Cytopathic effect was not seen during the period of time. In JaGar, maximum peak of the virus titre was reached on days of 35th, in the course of-´the 7 weeks culture and in HM-81, on days of 21st, during the 5 weeks culture. There may be some difference in infectivity between HM-81 and JaGar strains against the cells.
This result suggest that several type of cells might be clonizing for the purpose of the studying´ more detail.

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