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Ehrlich 腹水腫瘍 생쥐 各組織의 Insulin-I^(131) 및 RISA 分布容積에 關한 硏究

Insulin-I^(131) and RISA Spaces of Various Tissues of Mouse Bearing Ehrlich Ascites Tumor

대한생화학회잡지 1965년 2권 2호 p.15 ~ 23
이한구 (  ) - 서울대학교 의과대학 외과학교실

Abstract


For the purpose of measuring the insulin-I131 and RISA spaces, eighteen mice, that were already affected by Ehrich ascites tumor, were divided into two groups.
Each mouse of the first group was received 5,-10PC of insulin-I13´ through the tail vein. One hour after the injection the mice were sacrified and tumor tissue, renal cortex, cardiac muscle and skeletal muscle were excised from the body to determine the insulin-I131 space.
The mice of the second group were received 5-10PC of RISA respectively. One hour after injection, the tumor tissue and adjacent skin (as a control) were excised, then RISA space and Na space were measured.
The blood and tissue from the mice of the first group were treated with TCA and divided into TCA soluble and TCA precipitable fractions. Insulin-I13´ space of each tissue was calculated by isotope dilution principle obtaining the ratio between radioactivity of TCA precipitable fraction in the plasma and the tissue.
I13´ space was separately calculated with the ratio of radioactivity of TCA soluble fraction obtained from blood and each tissue in order to find the destruction rate of the insulin within each tissue.
In analyzing the result of second group; The blood volume of the tissue was obtained by the ratio of the specific activity (S.A) of RISA in the blood and tissue while Na space was calculated by the ratio between Na concentration in plasma and tissue.
The summary of the above experimental results were as follows:
1. In reviewing the first group; Insulin-I131 space
was 43.3-14.8% in the tumor tissue and approximately 30% in the liver, heart muscle and skeletal muscle, which were almost similar size of extracellular body fluid. From renal cortex, the value indicated about 115 %, the highest of all, but still it was only 1/5 when it was compared with value of normal animal. On the contrary distribution space of split products of injected insulin-I131, that was 113´ space, was approximately 50% in the liver, heart muscle and skeletal muscle, which were higher than the volume of extracellular fluid, and it was 116.4-28. 1% from tumor tissue which mean the closer concentration than in the case of plasma radioactivity. It was also high at renal cortex with 140.81 38.7%, but it was only 1/3 of the normal animal.
From the above data; Insulin-I331 space at tumor tissue was nearly similar in size with extracellular fluid while 1´31 space was larger at each tissue than extra-cellular fluid and especially tumor tissue was remarkably increased. Insulin was distributed in only extracellular fluid at tumor tissue and it was clearly shown that once insulin was introduced into the cell, it was easy to be destroyed. That was the fact led to an impression of the body insulin activity was generaly decreased by the tumor tissue.
2. When compared with control skin group 4.07-!-1.9% the blood volume of tumor tissue substantially decreased to 1.74-0. 67% at tumor tissue and generally speaking tumor tissue was almost placed at anoxic state. Na space was mean of 44.9-!-17.5% in the tumor tissue and 31. 0 -14.2% in the control group, revealing that it was rather higher in the tumor tissue.

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