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X - 선 전신조사동물의 장기에서 분리한 크로마틴에 관한 연구

Studies on the Chromatin Isolated from the Organs of Animals Received Whole - body X-ray Irradiation

대한수의학회지 1967년 7권 2호 p.13 ~ 18
한수남 (  ) - Seoul National University College of Agriculture

Abstract


1. Within experimental chromatin, the total protein: DNA ratio did not vary in the same organs of control and irradiated rats. However, the amount of RNA and total protein associated with the DNA varied considerably among the different types of chromatin.
In particular, the content of chromatin was the control tissue. RNA and total protein ratio of chromatins from brtain, liver, testis and spleen declined with experimental organs.
2. There was the same quantitative relationship between the amount of RNA and the amount of histone-protein associated with DNA in chromatin.
3. RNA: DNA ratio of chromatin allowed 1, 5-2 times increase in the irradiatedl organs except brain. However, RNA: DNA ratio was decreased in chromatin by irradiation.
4. Histone-protein:residual protein ratio was greatly varied among the organs. However, the effect was not found by irradiation.
5. Priming activity of chromatin showed a higher value in testis and the activity was greater in organs with higher metabolic activity.
6. Inhibition of Actinomycin D is observable in chromatin from testis, liver, spleen and brain declined without relationship between irradiated and non-irradiated conditions. Ammonium sulfate showed increased priming activity by the electrostatic dissociation of DNA and histone in chromatin on the stimulation depending on property of chromatins.
7. It is suggested that the results support a proposal that testis and spleen of highly sensitive to irradiation should an increase in the priming activity whereas brain and liver of lower sensitivity decreased in the activity.

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