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소 외안근 및 모양체에서 분리한 Mitochondria 분획내 Adenosinetriphosphatase 활성도에 미치는 Mg^(++), Ca^(++), Na^(+) 및 K^(+)의 영향

The Effects of Mg^(++), Ca^(++), Na^(+) 및 K^(+), on the ATPase Activityof Mitochondrial Fraction Isolated from Bovine xtraocular Muscle and Ciliary Process

대한안과학회지 1970년 11권 4호 p.23 ~ 32
박병국 (  ) - 부산대학교 의과대학 안과학교실

Abstract


The author investigated the effect of Mg++, Ca++, Na+ and K+ on the ATPase activity of mitochondrial fraction isolated from bovine extraocular muscles and ciliary processes and obtained. the following results:
1. The mitochondrial fraction isolated from bovine extrocoular muscle and ciliary process appeared to possess the Na+ & K+ activated ATPase in the presence of Mg++, and, on the contrary, in the absence of Mg++,the ATPase was not activated by K +,Na+ and Ca++.
2. The ATPase activity was enhanced with the increase of protein concentration in fractions from both, the bovine extraocular muscle and the ciliary process.
3. The ATPase in those fractions was potently activated under the condition of that the sample was modulated at 10 mM of K+ and 100mM of Na+, respectively.
4. The K+-and, Na+-activated ATPase in the presence of Mg++ was stimulated to enhance its activity by the addition of Ca up to 5mM, though by the higher concentration of Ca++ up to 10mM, its activity did not correspondingly increase.

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