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HeLa Cell 및 Amnion Cell 培養에 關하여

The Simple Procedure for the Cutivation of HeLa Cell and Amnion Cell

대한의학협회지 1964년 7권 4호 p.357 ~ 360
宋榮冕 (  ) - 서울大學校 微生物學敎室

朴鎭泳 (  ) - 서울大學校 微生物學敎室
李鎬汪 (  ) - 서울大學校 微生物學敎室

Abstract


It has long been established as routine procedure that supply of cell culture tube is needed to maintain the established cell lines and to assey virus andits neutralizing antibody. The present investigators have been able to simplify the method in culturing ctory results were obtained by utilizing growth media LYBSS-80, CoS-20 for Hela cells and LBSS-90, CoS-10 for amnion cells.
2. It is well to use two-ounce prescription bottle in tissue culture.
3. By determining the amount of 1.4 NaHC0_(3) tobe added to the growth media it is no longer necessary to adjust pH of growth media whenever it is freshly prepared.
4. Cell counting may not be needed each time since we are aware of proper composition of cell suspension.

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