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사람 호흡기 상피세포에서 Protopanaxadiol의 Lipopolysaccharide로 유도된 활성산소 생성과 MUC5AC 과발현 억제 효과

Inhibitory Effects of Protopanaxadiol on Lipopolysaccharide-Induced Reactive Oxygen Species Production and MUC5AC Expression in Human Airway Epithelial Cells

대한이비인후과학회지-두경부외과학 2019년 62권 9호 p.507 ~ 514
송유선 ( Song Yoo-Sun ) - 영남대학교 의과대학 이비인후-두경부외과학교실

김준희 ( Kim Joon-Hee ) - 영남대학교 의과대학 이비인후-두경부외과학교실
나형균 ( Na Hyung-Gyun ) - 영남대학교 의과대학 이비인후-두경부외과학교실
최윤석 ( Choi Yoon-Seok ) - 영남대학교 의과대학 이비인후-두경부외과학교실
송시연 ( Song Si-Youn ) - 영남대학교 의과대학 이비인후-두경부외과학교실
김용대 ( Kim Yong-Dae ) - 영남대학교 의과대학 이비인후-두경부외과학교실
배창훈 ( Bae Chang-Hoon ) - 영남대학교 의과대학 이비인후-두경부외과학교실

Abstract


Background and Objectives: MUC5AC is one of the major secretory mucin genes in the human airway epithelium. MUC5AC expression is increased by a variety of inflammatory mediators. Protopanaxadiol (PPD), one of the major active metabolites in ginseng, is known to have anti-inflammatory, antitumor and antioxidant properties. However, the effects of PPD on mucin secretion of airway epithelial cells still have not been reported. Therefore, the aim of this study is to investigate the effect of PPD on lipopolysaccharide (LPS)-induced MUC5AC expression in human airway epithelial cells.

Materials and Method: In the mucin-producing human NCI-H292 airway epithelial cells, the effect of PPD on MUC5AC expression was investigated using reverse transcription-polymerase chain reaction and enzyme immunoassay after treated with LPS. N-acetylcysteine (NAC) as a reactive oxygen species (ROS) scavenger, and apocynin as a nicotinamide adenine dinucleotide phosphate oxidase inhibitor were used to compare the inhibitory effect of PPD on LPSinduced ROS production in human NCI-H292 cells.

Results; LPS significantly increased MUC5AC mRNA expression and protein production. LPS also increased ROS production. PPD inhibited LPS-induced MUC5AC mRNA expression and protein production as well as ROS production. In addition, NAC and apocynin inhibited LPS-induced MUC5AC mRNA expression and protein production.

Conclusion: These results demonstrate that PPD inhibits LPS-induced MUC5AC expression via ROS in human airway epithelial cells and the inhibitory effect of PPD was similar to that of NAC and apocynin. These findings indicate that PPD may be a therapeutic agent for control of mucus secretion and oxidative stress in human airway epithelial cells.

키워드

Human airway epithelial cells; MUC5AC; Protopanaxadiol; Reactive oxygen species
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