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Streptococcus mutans에 대한 생강과 천연추출물의 광역학 항균효과

Antibacterial effect of Zingiberaceae extracts mediated photodynamic therapy on Streptococcus mutans

대한치과의사협회지 2019년 57권 10호 p.560 ~ 568
황혜림 ( Hwang Hye-Rim ) - 연세대학교 치과대학 예방치과학교실

강시묵 ( Kang Si-Mook ) - 연세대학교 치과대학 예방치과학교실
이은송 ( Lee Eun-Song ) - 연세대학교 치과대학 예방치과학교실
김백일 ( Kim Baek-Il ) - 연세대학교 치과대학 예방치과학교실

Abstract


Purpose: This study evaluated the antibacterial effects of curcuma, ginger, and finger root extracts in water-soluble powder on planktonic Streptococcus mutans (S.mutans ), which is known to cause dental caries, in order to confirm whether these extracts could perform as photosensitizers for the effects of photodynamic therapy (PDT).

Methods: This study used the strain of streptococcus mutans ATCC 25175 distributed by the Korean Collection for Type Cultures of the Korea Research Institute of Bioscience & Biotechnology. Commercial edible curcuma, ginger and finger root were used as the natural extracts for the use of photosensitizer.
To extract organic solvent, 3 g of each powder was mixed in 30 ㎖ of dimethyl sulfoxide (DMSO, VWR, Germany) before extraction. 1.8 ㎖ of the photosensitizer solution, manufactured in the concentrations of 5, 0.5, and 0.05 ㎎/㎖, was mixed with 0.2 ㎖ of the S. mutans culture medium that had been cultured for 2 days. To induce the photodynamic reaction, Qraycam (AIOBIO, Seoul, Korea) equipped with 405 ㎚ LED was used to expose light for 5 minutes to irradiate 59 ㎻ energy for 300 seconds.

Results: Compared with the case with no light, a higher photodynamic therapeutic effect was confirmed with 0.05㎎/㎖ curcuma powder extract, the concentration of 0.5 ㎎/㎖ and LED light of 405 ㎚ wavelength (p=0.000, p=0.003). 0.05 ㎎/㎖ of curcuma powder extract and the concentration of 0.5 ㎎/ ㎖ showed 100% antibacterial effect when exposed to light, whereas the concentration of 5 ㎎/㎖ showed 11.95% antibacterial effect. When exposed to light, 0.05 ㎎/㎖ of ginger powder extract showed an antibacterial effect which didn’t statistically decrease. The concentrations of 0.5 ㎎/㎖ and 5 ㎎/㎖ did not show any antibacterial effects. As a result of examining any photodynamic therapeutic effects of finger root powder extract on S. mutans , no statistically significant effect was found.

Conclusion: The curcuma powder extract is expected to perform as a photosensitizer. Even though belonging to the same ginger family, ginger powder and finger root powder seem difficult to perform as photosensitizer.

키워드

photodynamic therapy; natural photosensitizer; Quantitative Light-induced fluorescence
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