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The effect of human mesenchymal stem cell injection on pain behavior in chronic post-ischemia pain mice

Korean Journal of Pain 2020년 33권 1호 p.23 ~ 29
유시현 ( Yoo Sie-Hyeon ) - Soonchunhyang University College of Medicine Soonchunhyang University Cheonan Hospital Department of Anesthesiology and Pain Medicine

이성현 ( Lee Sung-Hyun ) - Sungkyunkwan University School of Medicine Kangbuk Samsung Hospital Department of Anesthesiology and Pain Medicine
이승환 ( Lee Seung-Hwan ) - Catholic University College of Medicine Seoul St. Mary’s Hospital Department of Anesthesia and Pain Medicine
박재홍 ( Park Jae-Hong ) - Catholic University College of Medicine Seoul St. Mary’s Hospital Department of Anesthesia and Pain Medicine
이승현 ( Lee Seung-Hyeon ) - Soonchunhyang University College of Medicine Soonchunhyang University Bucheon Hospital Department of Anesthesiology and Pain Medicine
진희철 ( Jin Hee-Cheol ) - Soonchunhyang University College of Medicine Soonchunhyang University Bucheon Hospital Department of Anesthesiology and Pain Medicine
박휴정 ( Park Hue-Jung ) - Catholic University College of Medicine Seoul St. Mary’s Hospital Department of Anesthesiology and Pain Medicine

Abstract


Background: Neuropathic pain (NP) is considered a clinically incurable condition despite various treatment options due to its diverse causes and complicated disease mechanisms. Since the early 2000s, multipotent human mesenchymal stem cells (hMSCs) have been used in the treatment of NP in animal models. However, the effects of hMSC injections have not been studied in chronic post-ischemia pain (CPIP) mice models. Here, we investigated whether intrathecal (IT) and intrapaw (IP) injections of hMSCs can reduce mechanical allodynia in CPIP model mice.

Methods: Seventeen CPIP C57/BL6 mice were selected and randomized into four groups: IT sham (n = 4), IT stem (n = 5), IP sham (n = 4), and IP stem (n = 4). Mice in the IT sham and IT stem groups received an injection of 5 μL saline and 2 × 104 hMSCs, respectively, while mice in the IP sham and IP stem groups received an injection of 5 μL saline and 2 × 105 hMSCs, respectively. Mechanical allodynia was assessed using von Frey filaments from pre-injection to 30 days post-injection. Glial fibrillary acidic protein (GFAP) expression in the spinal cord and dorsal root ganglia were also evaluated.

Results: IT and IP injections of hMSCs improved mechanical allodynia. GFAP expression was decreased on day 25 post-injection compared with the sham group. Injections of hMSCs improved allodynia and GFAP expression was decreased compared with the sham group.

Conclusions: These results suggested that hMSCs may be also another treatment modality in NP model by ischemia-reperfusion.

키워드

Ganglia; Spinal; Glial Fibrillary Acidic Protein; Hyperalgesia; Mesenchymal Stromal Cells; Mice; Neuralgia; Reperfusion Injury; Spinal Cord; Stem Cells
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