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Molecular Characterization of Clinically Isolated Staphylococcus aureus

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¿Àº¸¿µ ( Oh Bo-Young ) - ÀÎõº¸°Çȯ°æ¿¬±¸¿ø Áúº´Á¶»ç°ú

±èÁ¤Èñ ( Kim Jung-Hee ) - ÀÎõº¸°Çȯ°æ¿¬±¸¿ø Áúº´Á¶»ç°ú
°ø¿ë¿ì ( Gong Young-Woo ) - ÀÎõº¸°Çȯ°æ¿¬±¸¿ø Áúº´Á¶»ç°ú
ÀÌÁ¦¸¸ ( Lee Jae-Mann ) - ÀÎõº¸°Çȯ°æ¿¬±¸¿ø Áúº´Á¶»ç°ú
°íÁ¾¸í ( Go Jong-Myoung ) - ÀÎõº¸°Çȯ°æ¿¬±¸¿ø Áúº´Á¶»ç°ú
±è¿ëÈñ ( Kim Yong-Hee ) - ÀÎõº¸°Çȯ°æ¿¬±¸¿ø Áúº´Á¶»ç°ú

Abstract

Ȳ»öÆ÷µµ»ó±¸±ÕÀº ÁÖ¿äÇÑ ±âȸ °¨¿°±ÕÀ¸·Î, ÃÖ±Ù ¿©·¯ °¡Áö Ç×»ýÁ¦¿¡ ³»¼ºÀ» Áö´Ñ ¸ÞÄ¡½Ç¸° ³»¼º Ȳ»öÆ÷µµ»ó±¸±Õ(Methicillin-resistant Staphylococcus aureus: MRSA)ÀÌ ´Ã¾î³ª ¹®Á¦°¡ µÇ°í ÀÖ´Ù. ÀÌ¿¡ º» ¿¬±¸¿¡¼­´Â ÀÎõÁö¿ª°ü³» ¼³»çȯÀÚ¿¡¼­ ºÐ¸®ÇÑ Àåµ¶¼Ò ¾ç¼ºÀÎ S. aureus¸¦ ´ë»óÀ¸·Î Ç×»ýÁ¦ °¨¼ö¼º½ÃÇè ¹× PCRÀ» ÀÌ¿ëÇÑ tsst, eta, etb, mecA À¯ÀüÀÚ °Ë»ç¸¦ ½Ç½ÃÇÏ¿© »ý¹°ÇÐÀûÀΠƯ¼ºÀ» Á¶»çÇÏ¿´°í, tsst ¾ç¼ºÀÎ MRSA¸¦ ´ë»óÀ¸·Î Pulse Field Gel lectrophoresis (PFGE)¿¡ ÀÇÇÑ À¯ÀüÀÚÇüÀ» ºÐ¼®ÇÔÀ¸·Î¼­ °æ½ÃÀûÀÎ ºÐÀÚ¿ªÇÐÀû Ư¼ºÀ» ÆÄ¾ÇÇϰíÀÚ ÇÏ¿´´Ù. ±× °á°ú 2,281°ÇÀÇ ´ëº¯¿¡¼­ 173ÁÖÀÇ Àåµ¶¼Ò ¾ç¼ºÀÎ S. aureus¸¦ ºÐ¸®ÇÏ¿´À¸¸ç Aµ¶¼Ò¿Í Cµ¶¼Ò°¡ °¢°¢ 39%, 58%¸¦ Â÷ÁöÇÏ´Â °ÍÀ¸·Î ³ªÅ¸³µ´Ù. Ç×»ýÁ¦ °¨¼ö¼º °á°ú Àåµ¶¼Ò ¾ç¼ºÁÖ´Â ¸ðµÎ MRSA¿´À¸¸ç, ÀÌÁß 51%°¡ tsst ¾ç¼ºÀÎ °ÍÀ¸·Î ³ªÅ¸³µ°í eta, etb À¯ÀüÀÚ´Â °ËÃâµÇÁö ¾Ê¾Ò´Ù. mecA ³»¼ºÀ¯ÀüÀÚ´Â MRSA ±ÕÁÖÀÇ 97%°¡ ¾ç¼ºÀ¸·Î ³ªÅ¸³µ´Ù. tsst ¾ç¼ºÀÎ MRSA 88ÁÖ¸¦ ´ë»óÀ¸·Î PFGEÇÑ °á°ú, 10°³ÀÇ À¯ÇüÀ¸·Î ³ª´µ¾úÀ¸¸ç ±×Áß AÇü, HÇü ¹× FÇüÀÌ °¢°¢ 58%, 10%, 9%·Î ÁÖ¿äÇÑ ÇüÀ¸·Î ³ªÅ¸³µ´Ù.

Staphylococcus aureus is one of the most significant pathogens and a causative agents of nosocomial infections. The emergence of methicillin resistant S. aureus (MRSA), in particular, has become a major clinical and epidemiological problems worldwide. In this study, we analyzed the toxin genes and investigated molecular epidemiological characteristics of S. aureus isolated from stools of diarrheal patients at the hospitals in Incheon. Of the 609 strains from 2,281 specimens, 173 strains retained enterotoxin; 68 isolates (39.30%), 100 isolates (57.80%) were classified to A and C type, respectively. In the antibiotic susceptibility, all of enterotoxin positive isolates were resistant to oxacillin. Eighty eight strains (50.86%) of 173 MRSA isolate possessed tsst gene, but eta and etb genes were not detected at all. In the detection of MRSA associated genes by PCR method, mecA genes were detected in 167 strains (96.53%). From the result of PFGE analysis, we classified tsst-positive MRSA to 10 types and 24 subtypes. Type A, H and F were the major strains comprised of 57.95% (51strains), 10.22% (9 strains) and 9.09% (8 strains) respectively.

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antimicrobial resistance;MRSA;mecA;PFGE; tsst
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