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메뚜기의 내장에서 분리한 Moesziomyces 속에 속하는 셀룰로오스 분해 효모의 분리 및 특성

Isolation and characterization of cellulolytic yeast belonging to Moesziomyces sp. from the gut of Grasshopper

미생물학회지 2019년 55권 3호 p.234 ~ 241
김주영 ( Kim Ju-Young ) - 서울여자대학교 자연과학대학

정희영 ( Jung Hee-Young ) - 경북대학교 농업생명과학대학
박종석 ( Park Jong-Seok ) - 한국원자력연구원 첨단방사선연구소
조성진 (  ) - 충북대학교 자연과학대학 생명과학부
이훈복 ( Lee Hoon-Bok ) - 서울여자대학교 자연과학대학
성기호 ( Sung Gi-Ho ) - 가톨릭관동대학교 의과대학
가야쓰리 수브라마니 ( Gayathri Subramani ) - 서울여자대학교 자연과학대학
김명겸 ( Kim Myung-Kyum ) - 서울여자대학교 자연과학대학

Abstract


An intensive interaction between yeasts and insects has highlighted their relevance for attraction to food and for the insect's development and behavior. Yeast associated in the gut of insects secretes cellulase which aided in the food digestion (cellulose degradation). Three strains of cellulose-degrading yeast were isolated from the gut of adult grasshoppers collected in Gyeonggi Province, South Korea. The strains ON22 T , G10 T , and G15 T , showed positive cellulolytic activity in the carboxymethyl cellulose (CMC)-plate assay. The phylogenetic tree based on sequence analysis of D1/D2 domains of the large subunit rRNA gene and the internal transcribed spacer (ITS) regions revealed that the strains ON22 T (100 and 98.4% sequence similarities in D1/D2 domains and ITS) and G10 T (99.8 and 99.5% in D1/D2 domain and ITS region) were most closely related to the species Moesziomyces aphidis JCM 10318 T ; G15 T (100% in D1/D2 domains and ITS) belongs to the species Moesziomyces antarcticus JCM 10317 T , respectively.
Morphology and biochemical test results are provided in the species description. Cellulase with its massive applicability has been used in various industrial processes such as biofuels like bioethanol productions. Therefore, this is the first report of the cellulolytic yeast strains ON22 T , G10 T , and G15 T related to the genus Moesziomyces in the family Ustilaginaceae (Ustilaginales), in Korea.

키워드

Moesziomyces sp.; D1/D2 domain; grasshopper; ITS; rRNA; yeast
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