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인삼 재배 토양에서 분리한 진세노사이드 전환능력이 있는 Flavisolibacter ginsenosidimutans Gsoil 636 T의 유전체 서열 분석

Complete genome sequence of Flavisolibacter ginsenosidimutans Gsoil 636 T , a ginsenoside-converting bacterium, isolated from soil used for cultivating ginseng

미생물학회지 2019년 55권 4호 p.459 ~ 461
금동호 ( Keum Dong-Ho ) - 국립한경대학교 논업생명과학대학 생명공학과

이병희 ( Lee Byoung-Hee ) - 국립생물자원관 미생물자원과
이기은 ( Lee Ki-Eun ) - 국립생물자원관 미생물자원과
이순열 ( Lee Soon-Youl ) - 국립한경대학교 논업생명과학대학 생명공학과
임완택 ( Im Wan-Taek ) - 국립한경대학교 논업생명과학대학 생명공학과

Abstract


A yellow-colored, circular, convex, rod-shaped baterial strain designated Flavisolibacter ginsenosidimutans Gsoil 636 T was isolated from soil of a ginseng cultivation field in Pocheon Province, South Korea. Gsoil 636 T showed the ability to convert Rb1 (one of the dominant active components of ginseng) to F2, and its whole genome was sequenced. The whole genome of Flavisolibacter ginsenosidimutans Gsoil 636 T consist of a single circular chromosome of 5,079,621 bp, with 48.9% G + C content. Of the 4,338 predicted genes, 4,251 were proteincording genes, 46 were RNAs, and 41 were pseudogenes. Using the complete genome sequence of the strain Gsoil 636 T , we identified several glycoside hydrolase-encoding genes that may be involved in the conversion of major ginsenosides into minor ginsenosides and unexpectedly found antibiotic biosynthesis- encoding genes and excinuclease genes.

키워드

Flavisolibacter ginsenosidimutans; complete genome; ginseng soil; glycoside hydrolase; PacBio RS II
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