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Detection of MYD88 L265P in patients with lymphoplasmacytic lymphoma/Waldenstrom macroglobulinemia and other B-cell non-Hodgkin lymphomas

대한혈액학회지 2016년 51권 3호 p.181 ~ 186
신상용 ( Shin Sang-Yong ) - National Cancer Center Department of Laboratory Medicine

이승태 ( Lee Seung-Tae ) - Yonsei University College of Medicine Department of Laboratory Medicine
김현영 ( Kim Hyun-Young ) - Sungkyunkwan University School of Medicine Samsung Medical Center Department of Laboratory Medicine and Genetics
박창훈 ( Park Chang-Hun ) - Sungkyunkwan University School of Medicine Samsung Medical Center Department of Laboratory Medicine and Genetics
김희진 ( Kim Hee-Jin ) - Sungkyunkwan University School of Medicine Samsung Medical Center Department of Laboratory Medicine and Genetics
김종원 ( Kim Jong-Won ) - Sungkyunkwan University School of Medicine Samsung Medical Center Department of Laboratory Medicine and Genetics
김석진 ( Kim Seok-Jin ) - Sungkyunkwan University School of Medicine Samsung Medical Center Department of Internal Medicine
김원석 ( Kim Won-Seog ) - Sungkyunkwan University School of Medicine Samsung Medical Center Department of Internal Medicine
김선희 ( Kim Sun-Hee ) - Sungkyunkwan University School of Medicine Samsung Medical Center Department of Laboratory Medicine and Genetics

Abstract


Background: Recent studies have identified a high prevalence of the MYD88 L265P mutation in lymphoplasmacytic lymphoma (LPL)/Waldenstrom macroglobulinemia (WM) cases, whereas low frequencies have been observed in other B cell non-Hodgkin lymphomas (NHLs).

Methods: We evaluated the sensitivity of the mutant enrichment 3'-modified oligonucleotide (MEMO)-PCR technique, a new detection method. We examined the MYD88 L265P mutation in a series of Korean patients with LPL/WM and other B cell NHLs in bone marrow aspirates, using the MEMO-PCR technique.

Results: The sensitivity of MEMO-PCR was estimated to be approximately 10-16.7%. MYD88 L265P was detected in 21 of 28 LPL cases (75%) and only three of 69 B cell NHL cases (4.3%).

Conclusion: Although MEMO-PCR had relatively low sensitivity, we confirmed the high prevalence of the MYD88 L265P mutation in Korean LPL patients. Our study suggests the diagnostic value of MYD88 L265P for differentiating B-cell NHLs.

키워드

Lymphoplasmacytic lymphoma; MYD88 L265P; Aspirate; MEMO-PCR
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