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Saccharomyces cerevisiae 의 회분식 배양에서 세라마이드의 생산

The ceramide contents of Saccharomyces cerevisiae in batch culture

한국생물공학회지 2008년 23권 5호 p.449 ~ 451


Saccharomyces cerevisiae를 배양하여 ceramide를 생산하였다. 각기 다른 growth phase에서의 ceramide 생산량을 비교 실험하였다. Ceramide는 ELSD가 장착된 HPLC를 통하여 분석하였으며 stationary phase에 접어든 후 12시간 후에 회수, 추출된 양(2.01 mg ceramide/g cell)이 최대인 것으로 나타났다.

Ceramide has become a widely used ingredient in cosmetic and pharmaceutical industries, however, only a few yeast strains were investigated for the synthesis of ceramide and the concentration was very low. Ceramide is not only a core intermediate of sphingolipids but also an important modulator of many cellular events including apoptosis, cell cycle arrest, senescence, differentiation, and stress responses. In this study S.cerevisiae was grown in a batch culture and the cellular content of ceramide was measured at different growth phases. The ceramide content was highest at stationary phase and 2.01 mg ceramide/g cell was obtained.


Saccharomyces cerevisiae;ceramide;growth phases
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