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쌍별귀뚜라미 단백가수분해물의 제조 및 항산화 활성

Enzymatic preparation and antioxidant activities of protein hydrolysates from Gryllus bimaculatus

한국식품과학회지 2019년 51권 5호 p.473 ~ 479
조혜린 ( Cho Hye-Rin ) - 계명대학교 식품가공학과

이유정 ( Lee Yoo-Jung ) - 계명대학교 식품가공학과
홍지은 ( Hong Ji-Eun ) - 계명대학교 식품가공학과
이승욱 ( Lee Seung-Ook ) - 계명대학교 식품가공학과

Abstract


Gryllus bimaculatus (GB) has recently been registered as a food variety in Korea. In the present study, we prepared protein hydrolysates from GB and evaluated their antioxidant capacity. Protein hydrolysates were prepared from dried GB using enzymatic hydrolysis using five different proteases, and protein hydrolysates showing high hydrolysis value (alcalase, flavourzyme, and neutrase) were separated further into fractions ≤3 kDa and then lyophilized. Based on RC50 values of hydrolysates (≤3 kDa) obtained from four different antioxidant analyses, the flavourzyme hydrolysates showed relatively high levels of antioxidant capacity among the three hydrolysates, and in particular, it showed considerably strong antioxidant activity in 2,2-diphenyl-1-picrylhydrazyl (DPPH) assays. The flavourzyme hydrolysate also significantly inhibited peroxidation of linoleic acid. These results suggest that protein hydrolysates from GB represent potential sources of natural antioxidants. Our current studies are focused on identification of active peptides from the flavourzyme hydrolysate.

키워드

Gryllus bimaculatus; protein hydrolysates; flavourzyme; peptide; antioxidant
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