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싸리나무 추출물의 Xanthoangelol 분리 및 HPLC-DAD 밸리데이션

Isolation and HPLC-DAD validation of xanthoangelol in Lespedeza bicolor extract

한국식품과학회지 2020년 52권 1호 p.26 ~ 30
우현심 ( Woo Hyun-Sim ) - 국립백두대간수목원 산림식물산업부실

김영수 ( Kim Yeong-Su ) - 국립백두대간수목원 산림식물산업부실
오유진 ( Oh Yu-Jin ) - 국립백두대간수목원 산림식물산업부실
조해진 ( Cho Hae-Jin ) - 국립백두대간수목원 산림식물산업부실
송세규 ( Song Se-Kyu ) - 국립백두대간수목원 산림식물산업부실
김대욱 ( Kim Dae-Wook ) - 국립백두대간수목원 산림식물산업부실

Abstract


This study was undertaken to determine the characteristics of xanthoangelol, the major chalcone constituent derived from the extracts of different parts of Lespedeza bicolor. Xanthoangelol was isolated from the root extract using column chromatography and used as a standard for quantitative analysis. The structure of the isolated compound was established based on spectroscopic evidence. The HPLC-DAD method was validated for specificity, linearity, precision, accuracy, limit of detection, and limit of quantitation. The calibration curve of xanthoangelol had significant linearity (R2>0.9999). Limit of detection and limit of quantitation 0.018 and 0.059 μg/mL, respectively. The relative standard deviation values of precision test, and intra- and inter-day tests were less than 0.22 and 0.40%, respectively. In the recovery test, the accuracy ranged from 98.98-102.78% with RSD values less than 0.13%. The method validation parameters indicate the applicability of the HPLC method for quality control of food or drug formulations containing L. bicolor.

키워드

Lespdeza bicolor; chalcone; xanthoangelol; HPLC-DAD; validation
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