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Analysis of longissimus muscle quality characteristics and associations with DNA methylation status in cattle

Genes & Genomics 2019년 41권 10호 p.1147 ~ 1163
 ( Chen Zhi ) - Yangzhou University College of Animal Science and Technology

 ( Chu Shuangfeng ) - Yangzhou University College of Animal Science and Technology
 ( Xu Xin ) - Yangzhou University College of Animal Science and Technology
 ( Jiang Jingyi ) - Yangzhou University College of Animal Science and Technology
 ( Wang Wenqiang ) - Yangzhou University College of Animal Science and Technology
 ( Shen Hongliang ) - Animal Health Inspection
 ( Li Mingxun ) - Yangzhou University College of Animal Science and Technology
 ( Zhang Huimin ) - Yangzhou University College of Animal Science and Technology
 ( Mao Yongjiang ) - Yangzhou University College of Animal Science and Technology
 ( Yang Zhangping ) - Yangzhou University College of Animal Science and Technology

Abstract


Background: As cattle represent one of the most important livestock species for meat production, control of muscle development in regards to quality is an important research focus.

Objectives: In this study, the phenotypic quality traits and its associations with DNA methylation levels of the longissimus muscle in two cattle breeds were studied.

Methods: The pH value, water loss rate, fat and protein and fatty acid content were measured in three beef cattle breeds of longissimus mucle; The longissimus mucle was analyzed by MethylRAD-seq and RNA-seq. The differentially methylated and differentially expressed related genes were subjected to BSP.

Results: Methylation status of longissimus mucle was analyzed by MethylRAD-seq. Compared with Simmental, there were 39 differentially methylated and expressed genes in muscle of Yunling cattle, and 123 differentially methylated and expressed genes in Wenshan muscle. A combined analysis of MethylRAD-seq and RNA-seq results revealed differential methylation and expression level of 18 genes between Simmental and Wenshan cattle, and 14 genes between Simmental and Yunling cattle. In addition, 28 genes were differentially methylated between Wenshan and Yunling cattle. Results of promoter methylation analysis of ACAD11, FADS6 and FASN showed that the overall degree of DNA methylation of FADS6 and FASN was negatively correlated with their expression levels. Methylation level of FASN in Simmental was greater than Yunling and Wenshan. The degree of methylation at the FADS6 CpG4 site was significantly higher in Simmental than that in Yunling. The levels of methylation at the CpG7 locus of the Simmental and Yunling breeds were greater than Wenshan cattle. A negative correlation was detected between the methylation levels and the expression of FASN CpG1, CpG2, CpG3, CpG5, CpG7, and CpG10.

Conclusion: The functional and molecular regulatory mechanism of the genes related to meat quality can be revealed systematically from aspects of the genetic and epigenetic regulation. These studies will help to further explore the molecular mechanisms and phenotypic differences that regulate growth and quality of different breeds of cattle.

키워드

Meat quality; MethylRAD-seq; BSP; Cattle
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