medric medric
[닫기]
잠시만 기다려 주세요. 로딩중입니다.

Association analysis of polymorphism in the NR6A1 gene with the lumbar vertebrae number traits in sheep

Genes & Genomics 2019년 41권 10호 p.1165 ~ 1171
 ( Zhang Xiangyu ) - Shihezi University College of Life Science

 ( Li Cunyuan ) - Shihezi University College of Life Science
 ( Li Xiaoyue ) - Shihezi University College of Life Science
 ( Liu Zhijin ) - Shihezi University College of Life Science
 ( Ni Wei ) - Shihezi University College of Life Science
 ( Cao Yang ) - Shihezi University College of Life Science
 ( Yao Yang ) - Shihezi University College of Life Science
 ( Islamov Esenbay ) - Kazakh National Agrarian University
 ( Wei Junchang ) - Shihezi University College of Life Science
 ( Hou Xiaoxu ) - Shihezi University College of Life Science
 ( Hu Shengwei ) - Shihezi University College of Life Science

Abstract


Introduction: The vertebral number is an economically significant trait, which is associated with body length and carcass traits. Nuclear Receptor Subfamily 6, Group A, Member 1 (NR6A1) is a member of the nuclear receptor superfamily and it plays an important role in the early development of embryos.

Objectives: The NR6A1 gene was considered as an important candidate for influence vertebrae number, while the potential associations between this gene and the number of lumbar vertebrae traits of sheep have not been explored.

Methods: In this study, we detected the genetic variants of NR6A1 gene and analyzed the associations of the polymorphisms with lumbar number traits in 130 Kazakh sheep. We use single-strand conformation polymorphism (SSCP) technique to detect single nucleotide polymorphism (SNP) of NR6A1 gene, and the association of the genotype and lumbar number variation was analyzed by independent Chi-square test.

Results: We detect SNP of NR6A1 gene by PCR-SSCP technique, and polymorphisms were only found in the coding region of exon-6 and exon-8 of NR6A1 gene. In order to investigate the connection between the SNP locus and lumbar number traits in sheep, we conducted a Chi-square test for independence for exon-6 and exon-8 of NR6A1 gene, respectively. Association analysis revealed significant associations between the SNP (rs414302710: A?>C) in the exon-8 of NR6A1 gene with the number of lumbar vertebrae (P?
Conclusion: Our study indicated that this SNP (rs414302710: A>C) locus of exon-8 of NR6A1 gene in sheep possible influence the number of lumbar vertebrae, which has the potential to be applied in selective breeding of sheep.

키워드

NR6A1 gene; SSCP; SNP; Lumbar vertebrae
원문 및 링크아웃 정보
 
등재저널 정보