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Physiological responses and small RNAs changes in maize under nitrogen deficiency and resupply

Genes & Genomics 2019년 41권 10호 p.1183 ~ 1194
 ( Yang Zhenchao ) - Northwest A&F University College of Life Sciences

 ( Wang Zhengyan ) - Northwest A&F University College of Life Sciences
 ( Yang Chengcheng ) - Northwest A&F University College of Life Sciences
 ( Yang Zhao ) - Northwest A&F University College of Life Sciences
 ( Li Hongquan ) - Northwest A&F University College of Life Sciences
 ( Wu Yongjun ) - Northwest A&F University College of Life Sciences


Background: Maize is an important crop in the world, nitrogen stress severely reduces maize yield. Although a large number of studies have identified the expression changes of microRNAs (miRNAs) under N stress in several species, the miRNAs expression patterns of N-deficient plants under N resupply remain unclear.

Objective: The primary objective of this study was to identify miRNAs in response to nitrogen stress and understand relevant physiological changes in nitrogen-deficient maize after nitrogen resupply.

Methods: Physiological parameters were measured to study relevant physiological changes under different nitrogen conditions. Small RNA sequencing and qRT-PCR analysis were performed to understand the response of miRNAs under different nitrogen conditions.

Results: The content of chlorophyll, soluble protein and nitrate nitrogen decreased than CK by 0.52, 0.49 and 0.82 times after N deficiency treatment and increased than ND by 0.52, 1.36 and 0.65 times after N resupply, respectively. Conversely, the activity of superoxide dismutase (SOD) and peroxidase (POD) increased by 0.67 and 1.64 times than CK after N deficiency, respectively, and decreased by 0.09 and 0.35 times than ND after N resupply. A total of 226 known miRNAs were identified by sRNA sequencing; 106 miRNAs were differentially expressed between the control and N-deficient groups, and 103 were differentially expressed between the N-deficient and N-resupply groups (P?
Conclusion: Our study comprehensively provides important information on miRNA functions and molecular mechanisms in response to N stress. These findings may assist to improve nitrogen availability in plants.


Maize; miRNA; Nitrogen resupply; Small RNA sequencing
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