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(Cyto)genomic and epigenetic characterization of BICR 10 cell line and three new established primary human head and neck squamous cell carcinoma cultures

Genes & Genomics 2019년 41권 10호 p.1207 ~ 1221
 ( Ribeiro Ilda P. ) - University of Coimbra Faculty of Medicine Cytogenetics and Genomics Laboratory

 ( Rodrigues Joana M. ) - University of Coimbra Faculty of Medicine Cytogenetics and Genomics Laboratory
 ( Mascarenhas Alexandra ) - University of Coimbra Faculty of Medicine Cytogenetics and Genomics Laboratory
 ( Marques Vanessa ) - University of Coimbra Faculty of Medicine Cytogenetics and Genomics Laboratory
 ( Caramelo Francisco ) - University of Coimbra IBILI-Faculty of Medicine Laboratory of Biostatistics and Medical Informatics
 ( Juliao Maria J. ) - CHUC Coimbra Hospital and University Centre Department of Patholog
 ( Liehr Thomas ) - Friedrich Schiller University Jena University Hospital Institute of Human Genetics
 ( Melo Joana B. ) - University of Coimbra Faculty of Medicine Cytogenetics and Genomics Laboratory
 ( Carreira Isabel M. ) - University of Coimbra Faculty of Medicine Cytogenetics and Genomics Laboratory

Abstract


Background: Head and neck squamous cell carcinoma cell lines are useful preclinical models to understand the molecular processes underlying the development of such tumors, and to establish targeted therapies.

Objective: We performed a comprehensive (cyto)genomic and epigenetic characterization of three new established primary human head and neck squamous cell carcinoma cultures and an established, yet undercharacterized cell line: BICR 10.

Methods: Karyotyping, multiplex fluorescence in situ hybridization, array comparative genomic hybridization and methylation-specific multiplex ligation-dependent probe amplification were applied.

Results: The three primary cultures turned out to be a near-triploid and BICR 10 near-diploid. Banding and molecular cytogenetic analysis revealed non-random numerical and structural aberrations. The most common rearrangements identified in BICR 10 cell line were non-complex derivatives of reciprocal translocations, in which the breakpoints often appeared in centromeric/near-centromeric regions. In the 3 primary cell cultures the most common rearrangements observed were iso- and derivatives chromosomes derived from translocations. Overall, gains of 7p, 8q and losses at 3p, 8p, 9p, 18q and Xp were present in all four studied samples. Among the analyzed genes, BICR 10 cell line exhibited enhanced methylation of gene promoter; however, in all studied samples PAX5, WT1 and GATA5 were methylated.

Conclusion: The here reported comprehensive characterization of BICR 10 cell line and the new established cultures enriches the resources available for head and neck cancer research, especially for testing therapeutic agents.

키워드

Primary cell cultures; BICR 10 cell line; Head and neck cancer; Chromosomal rearrangements; Methylation
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