medric medric
[닫기]
잠시만 기다려 주세요. 로딩중입니다.

Simultaneous determination of ampicillin sodium and sulbactam sodium in powder for injection by HPLC

분석과학 2019년 32권 4호 p.147 ~ 154
 ( Mai Xuan-Lan ) - Kangwon National University College of Pharmacy

 ( Pham Thuy-Vy ) - Kangwon National University College of Pharmacy
한가현 ( Han Ga-Hyun ) - Kangwon National University College of Pharmacy
금수진 ( Kum Su-Jin ) - Kangwon National University College of Pharmacy
우상훈 ( Woo Sang-Hoon ) - Kangwon National University College of Pharmacy
 ( Kang Jong-Seong ) - Chungnam National University College of Pharmacy
 ( Woo Mi-Hee ) - Catholic University of Daegu College of Pharmacy
 ( Na Dong-Hee ) - Chungang University College of Pharmacy
전인구 ( Chun In-Koo ) - Kangwon National University College of Pharmacy
전인구 ( Chun In-Koo ) - Kangwon National University College of Pharmacy
 ( Kim Kyeong-Ho ) - Kangwon National University College of Pharmacy

Abstract


Ampicillin and Sulbactam (2:1, w/w) are combined in formulation to provide broader antibacterial action in treatment of many infections. The development of analytical method for simultaneouly determine these two compounds was difficult because of the differences in their chemical structures and ratio in the formulation.
Current published methods still have some limitations. In this study, we developed an alternative highperformance liquid chromatography (HPLC) assay method for simultaneously determination of ampicillin sodium and sulbactam sodium in powder for injection. Method validation of HPLC method was conducted to determine linearity, precision, accuracy, system suitability, robustness. The linearity of the calibration curves in the desired concentration range was good (r2 > 0.9994). RSDs of intra-day and inter-day precision obtained were less than 2.00 %. Accuracy was obtained with the recoveries in range of 98.42 % and 101.36 %. As a result of system suitability, RSD of both retention time and the peak area obtained were not more than 1.0 %. The values of plate number were more than 7000 and symmetric factors obtained were 0.8. As intermediate-precision and robustness of the developed assay, it could be expected to become valuable tools for revising the Korean Pharmacopoeia (KP XI).

키워드

high-performance liquid chromatography (HPLC); Korean Pharmacopoeia (KP XI); ampicillin sodium; sulbactam sodium; assay
원문 및 링크아웃 정보
등재저널 정보
KCI