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SH-SY5Y 세포에서 도네페질과 병용투여시 신경보호 효과를나타내는 한약재의 in vitro 선별 연구

In Vitro Screening of Traditional Medicinal Herbs Combined with Donepezil for Neuroprotective Effects in SH-SY5Y Cells

동의신경정신과학회지 2019년 30권 3호 p.199 ~ 208
송수진 ( Song Sue-Jin ) - 동국대학교 한의과대학 한방신경정신과학교실

류천봉 ( Liu Quan Feng ) - 동국대학교 한의과대학 한방신경정신과학교실
홍민호 ( Hong Min-Ho ) - 동국대학교 한의과대학 한방신경정신과학교실
김근우 ( Kim Geun-Woo ) - 동국대학교 한의과대학 한방신경정신과학교실
구병수 ( Koo Byung-Soo ) - 동국대학교 한의과대학 한방신경정신과학교실

Abstract


Objectives: The purpose of this study was to evaluate the neuroprotective effects of donepezil and 33 kinds of herbal extract combinations in SH-SY5Y cells with Aβ 25?35 treatment.

Methods: MTT assay was performed to measure the cell viability of each herbal extract combined with donepezil against Aβ-induced neurotoxicity. The most active extracts were then subjected to assess the effects on CREB phosphorylation and COX-2 expressions through the western blot analysis.

Results: There were eight herbal extracts representing significant increase on the cell viability: 1) Erycibe obtusifolia, 2) Polygonum multiflorum, 3) Polygala tenuifolia, 4) Illicium verum, 5) Santalum album, 6) Loranthus parasticus, 7) Platycladus orientalis, and 8) Zanthoxylum piperitum. Especially, when Santalum album and donepezil were treated together, the phosphorylation of CREB significantly increased and COX-2 protein expression was significantly inhibited.

Conclusions: Among the screened herbal extracts, combination treatment of each of the eight herbs and donepezil showed neuroprotective effects in SH-SY5Y cells. Additionally, the combination of Santalum album and donepezil suggested cognitive improvement by up-regulation of p-CREB and down-regulation of COX-2.

키워드

Herbal medicine; Donepezil; Drug combinations; Alzheimer’s disease
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