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Scopolamine 유발 기억력 손상 마우스 모델에서 분심기음의항산화 및 기억력 감퇴 억제 효과

Anti-amnesic and Antioxidant Effect of Bunsimgieum (Fenxinqiyin ) on Scopolamine-Induced Memory Impairment in Mic

동의신경정신과학회지 2019년 30권 3호 p.221 ~ 235
한다영 ( Han Da-Young ) - 대구한의대학교 한의과대학 신경정신과교실

유옥철 ( Yu Ok-Cheol ) - 자생한방병원 신경정신과
김상호 ( Kim Sang-Ho ) - 대구한의대학교 한의과대학 신경정신과교실
정대규 ( Chung Dae-Kyoo ) - 대구한의대학교 한의과대학 신경정신과교실

Abstract


Objectives: The purpose of this study was to confirm the anti-amnesic effects of Bunsimgieum (BSGE) through its favorable acetylcholine (ACh) and, acetylcholinesterase (AChE) activity, choline acetyltransferase (ChAT) mRNA expressions, and antioxidant effect on scopolamine (Sco)-induced memory impairment in C57BL/6 mice.

Methods: Six groups, a total of 20 intact or 100 Sco-induced mice were used in this study, based on their body weight. Half of each group underwent passive avoidance tests and the measurement of hippocampus AChE activity, ACh content, and ChAT mRNA expression, The remaining half of each group underwent a Morris water-maze test and antioxidant defense system measurement as well.

Results: Significant reductions in the step-through latency times from the passive avoidance test and reductions in the escape latency times from the Morris water-maze test were observed with increases of hippocampal AChE activities and, reductions in ACh contents and ChAT mRNA expression in hippocampus, as a result of Sco intraperitoneal treatment, in this study. Additionally, the increases in cerebral cortical MDA levels and, reductions in GSH contents, SOD activities, and CAT activities were demonstrated in the Sco control mice compared with the intact vehicle control mice, respectively. However, 28 days of consecutive oral pre-treatment of BSGE hot water extracts of 400, 200, and 100 mg/kg, respectively, markedly and dose-dependently inhibited Sco treatment-related amnesia.

Conclusions: The results demonstrate that the oral administration of BSGE hot water extracts reduces Sco-induced memory impairment, through preserving ACh, related to ChAT mRNA expressions, causes AChE inhibition, and enhances the cerebral antioxidant defense system.

키워드

Bunsimgieum (Fenxinqiyin); Scopolamine, Memory impairment; ACh; AChE; ChAT mRNA; Cerebral antioxidant defense system
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