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Anti-Biofilm Activity of Grapefruit Seed Extract against Staphylococcus aureus and Escherichia coli

Journal of Microbiology and Biotechnology 2019년 29권 8호 p.1177 ~ 1183
 ( Song Ye-Ji ) - Konkuk University Department of Food science and Biotechnology of Animal Resources

 ( Yu Hwan-Hee ) - Konkuk University Department of Food Science and Biotechnology of Animal Resources
 ( Kim Yeon-Jin ) - Konkuk University Department of Food science and Biotechnology of Animal Resources
 ( Lee Na-Kyoung ) - Konkuk University Department of Food science and Biotechnology of Animal Resources
 ( Paik Hyun-Dong ) - Konkuk University Department of Food science and Biotechnology of Animal Resources

Abstract


Grapefruit seed extract (GSE) is a safe and effective preservative that is used widely in the food industry. However, there are few studies addressing the anti-biofilm effect of GSE. In this study, the anti-biofilm effect of GSE was investigated against biofilm-forming strains of Staphylococcus aureus and Escherichia coli. The GSE minimum inhibitory concentration (MIC) for S. aureus and E. coli were 25 μg/ml and 250 μg/ml, respectively. To investigate biofilm inhibition and degradation effect, crystal violet assay and stainless steel were used. Biofilm formation rates of four strains (S. aureus 7, S. aureus 8, E. coli ATCC 25922, and E. coli O157:H4 FRIK 125) were 55.8%, 70.2%, 55.4%, and 20.6% at 1/2 × MIC of GSE, respectively. The degradation effect of GSE on biofilms attached to stainless steel coupons was observed (≥ 1 log CFU/coupon) after exposure to concentrations above the MIC for all strains and 1/2 × MIC for S. aureus 7. In addition, the specific mechanisms of this anti-biofilm effect were investigated by evaluating hydrophobicity, auto-aggregation, exopolysaccharide (EPS) production rate, and motility. Significant changes in EPS production rate and motility were observed in both S. aureus and E. coli in the presence of GSE, while changes in hydrophobicity were observed only in E. coli. No relationship was seen between auto-aggregation and biofilm formation. Therefore, our results suggest that GSE might be used as an anti-biofilm agent that is effective against S. aureus and E. coli.

키워드

Anti-biofilm; grapefruit seed extract; biofilm; Staphylococcus aureus; Escherichia coli
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