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Protective Effect of 3-Bromo-4,5-Dihydroxybenzaldehyde from Polysiphonia morrowii Harvey against Hydrogen Peroxide-Induced Oxidative Stress In Vitro and In Vivo

Journal of Microbiology and Biotechnology 2019년 29권 8호 p.1193 ~ 1203
 ( Cho Su-Hyeon ) - Korea Basic Science Institute Chuncheon Center

 ( Heo Soo-Jin ) - Korea Institute of Ocean Science and Technology
 ( Yang Hye-Won ) - Jeju National University Department of Marine Life Science
 ( Ko Eun-Yi ) - Dermapro
정명선 ( Jung Myeong-Seon ) - Korea Basic Science Institute Chuncheon Center
 ( Cha Seon-Heui ) - Hanseo University Department of Marine Biomedical Science
 ( Ahn Gin-Nae ) - Chonnam National University Department of Marine Bio-Food Sciences
 ( Jeon You-Jin ) - Jeju National University Department of Marine Life Science
 ( Kim Kil-Nam ) - Korea Basic Science Institute Chuncheon Center

Abstract


We investigated the protective effects of 3-bromo-4,5-dihydroxybenzaldehyde (BDB) from Polysiphonia morrowii Harvey against hydrogen peroxide (H2O2)-induced apoptosis in Vero cells. BDB exhibited scavenging activity for DPPH, hydroxyl, and alkyl radicals. BDB also inhibited H2O2-induced lipid peroxidation, cell death, and apoptosis in Vero cells by inhibiting the production of ROS. To evaluate the molecular mechanisms of apoptosis inhibition, the expression of Bax/Bcl-xL and NF-κB was assessed by western blot assay. BDB significantly suppressed the cleavage of caspase-9 and PARP and reduced Bax levels in H2O2-induced Vero cells. Besides, BDB suppressed the phosphorylation of NF-κB and the translocation of p65 in H2O2-induced cells. Furthermore, we evaluated the effect of BDB on ROS production, cell death, and lipid peroxidation in an H2O2-stimulated zebrafish embryo model. Taken together, these results indicated that ROS generation and cell death were significantly inhibited by BDB in zebrafish embryos, thereby proving that BDB exerts excellent antioxidant activity in vitro and in vivo.

키워드

Polysiphonia morrow Harvey; 3-bromo-4,5-dihydroxybenzaldehyde; reactive oxygen species; oxidative stress; antioxidant; zebrafish embryos
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