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1H-NMR-Based Metabolic Profiling of Cordyceps militaris to Correlate the Development Process and Anti-Cancer Effect

Journal of Microbiology and Biotechnology 2019년 29권 8호 p.1212 ~ 1220
 ( Oh Jun-Sang ) - Chung-Ang University College of Pharmacy

 ( Choi Eun-Hyun ) - Kainos Medicine Inc.
 ( Yoon Deok-Hyo ) - Catholic Kwandong University College of Medicine International St. Mary’s Hospital Translational Research Division
 ( Park Tae-Yong ) - Catholic Kwandong University College of Medicine International St. Mary’s Hospital Translational Research Division
 ( Shrestha Bhushan ) - Mushtech Cordyceps Institute
 ( Choi Hyung-Kyoon ) - Chung-Ang University College of Pharmacy
 ( Sung Gi-Ho ) - Catholic Kwandong University College of Medicine International St. Mary’s Hospital Translational Research Division

Abstract


The study of metabolomics in natural products using the diverse analytical instruments including GC-MS, LC-MS, and NMR is useful for the exploration of physiological and biological effects and the investigation of drug discovery and health functional foods. Cordyceps militaris has been very attractive to natural medicine as a traditional Chinese medicine, due to its various bioactive properties including anti-cancer and anti-oxidant effects. In this study, we analyzed the metabolite profile in 50% ethanol extracts of C. militaris fruit bodies from three development periods (growth period, matured period, and aging period) using 1H-NMR, and identified 44 metabolites, which are classified as 16 amino acids, 10 organic acids, 5 carbohydrates, 3 nucleotide derivatives, and 10 other compounds. Among the three development periods of the C. militaris fruit body, the aging period showed significantly higher levels of metabolites including cordycepin, mannitol (cordycepic acid), and β-glucan. Interestingly, these bioactive metabolites are positively correlated with antitumor growth effect; the extract of the aging period showed significant inhibition of HepG2 hepatic cancer cell proliferation. These results showed that the aging period during the development of C. militaris fruit bodies was more highly enriched with bioactive metabolites that are associated with cancer cell growth inhibition.

키워드

Cordyceps militaris; metabolomics; cordycepin; β-glucan; nuclear magnetic resonance; anti-cancer effect
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