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Proteomic and Morphologic Evidence for Taurine-5-Bromosalicylaldehyde Schiff Base as an Efficient Anti-Mycobacterial Drug

Journal of Microbiology and Biotechnology 2019년 29권 8호 p.1221 ~ 1229
 ( Ding Wenyong ) - Dalian Medical University College of Basic Medical Sciences Biochemistry and Molecular Biology Department

 ( Zhang Houli ) - Dalian Medical University Institute of Pharmacology
 ( Xu Yuefei ) - Dalian Medical University College of Basic Medical Sciences Biochemistry and Molecular Biology Department
 ( Ma Li ) - Dalian Medical University Department of Epidemiology
 ( Zhang Wenli ) - Dalian Medical University College of Basic Medical Sciences Biochemistry and Molecular Biology Department

Abstract


Mycobacterium tuberculosis, a causative pathogen of tuberculosis (TB), still threatens human health worldwide. To find a novel drug to eradicate this pathogen, we tested taurine-5- bromosalicylaldehyde Schiff base (TBSSB) as an innovative anti-mycobacterial drug using Mycobacterium smegmatis as a surrogate model for M. tuberculosis. We investigated the antimicrobial activity of TBSSB against M. smegmatis by plotting growth curves, examined the effect of TBSSB on biofilm formation, observed morphological changes by scanning electron microscopy and transmission electron microscopy, and detected differentially expressed proteins using two-dimensional gel electrophoresis coupled with mass spectrometry. TBSSB inhibited mycobacterial growth and biofilm formation, altered cell ultrastructure and intracellular content, and inhibited cell division. Furthermore, M. smegmatis adapted itself to TBSSB inhibition by regulating the metabolic pathways and enzymatic activities of the identified proteins. NDMA-dependent methanol dehydrogenase, NAD(P)H nitroreductase, and amidohydrolase AmiB1 appear to be pivotal factors to regulate the M. smegmatis survival under TBSSB. Our dataset reinforced the idea that Schiff base-taurine compounds have the potential to be developed as novel anti-mycobacterial drugs.

키워드

Mycobacterium; tuberculosis; taurine-5-bromosalicylaldehyde Schiff base; morphology; cell wall; two dimensional gel electrophoresis
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