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LasR Might Act as an Intermediate in Overproduction of Phenaz in the Absence of RpoS in Pseudomonas aeruginosa

Journal of Microbiology and Biotechnology 2019년 29권 8호 p.1299 ~ 1309
 ( He Qiuning ) - Ludong University School of Life Sciences Department of Applied and Environmental Microbiology

 ( Feng Zhibin ) - Ludong University School of Life Sciences Department of Applied and Environmental Microbiology
 ( Wang Yanhua ) - Ludong University School of Life Sciences Department of Applied and Environmental Microbiology
 ( Wang Kewen ) - Ludong University School of Life Sciences Department of Applied and Environmental Microbiology
 ( Zhang Kailu ) - Ludong University School of Life Sciences Department of Applied and Environmental Microbiology
 ( Kai Le ) - Ludong University School of Life Sciences Department of Applied and Environmental Microbiology
 ( Zhao Xiuying ) - Xinjiang Academy of Agricultural Sciences Institute of Applied Microbiology
 ( Yu Zhifen ) - Ludong University Affiliated Hospital
 ( Chen Lijuan ) - Ludong University Affiliated Hospital
 ( Ge Yihe ) - Ludong University School of Life Sciences Department of Applied and Environmental Microbiology

Abstract


As an opportunistic bacterial pathogen, Pseudomonas aeruginosa PAO1 contains two phenazineproducing gene operons, phzA1B1C1D1E1F1G1 (phz1) and phzA2B2C2D2E2F2G2 (phz2), each of which is independently capable of encoding all enzymes for biosynthesizing phenazines, including phenazine-1-carboxylic acid and its derivatives. Other previous study reported that the RpoS-deficient mutant SS24 overproduced pyocyanin, a derivative of phenazine-1- carboxylic acid. However, it is not known how RpoS mediates the expression of two phz operons and regulates pyocyanin biosynthesis in detail. In this study, with deletion of the rpoS gene in the PAΔphz1 mutant and the PAΔphz2 mutant respectively, we demonstrated that RpoS exerted opposite regulatory roles on the expression of the phz1and phz2 operons. We also confirmed that the phz1 operon played a critical role and especially biosynthesized much more phenazines than the phz2 operon when the rpoS gene was knocked out in P. aeruginosa. By constructing the translational reporter fusion vector lasR’-’lacZ and the chromosomal fusion mutant PAΔlasR::lacZ, we verified that RpoS deficiency caused increased expression of lasR, a transcription regulator gene in a first quorum sensing system (las) that activates overexpression of the phz1 operon, suggesting that in the absence of RpoS, LasR might act as an intermediate in overproduction of phenazine biosynthesis mediated by the phz1 operon in P. aeruginosa.

키워드

Pseudomonas aeruginosa; RpoS; LasR; phz1; phz2; Phenazine
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