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Intestinal Microbial Dysbiosis in Beagles Naturally Infected with Canine Parvovirus

Journal of Microbiology and Biotechnology 2019년 29권 9호 p.1391 ~ 1400
 ( Park Jun-Seok ) - Chungnam National University College of Veterinary Medicine

 ( Guevarra Robin B. ) - Dankook University Department of Animal Resources Science
 ( Kim Bo-Ra ) - Dankook University Department of Animal Resources Science
 ( Lee Jun-Hyung ) - Dankook University Department of Animal Resources Science
 ( Lee Sun-Hee ) - Dankook University Department of Animal Resources Science
 ( Cho Jae-Hyoung ) - Dankook University Department of Animal Resources Science
 ( Kim Hye-Ri ) - Dankook University Department of Animal Resources Science
 ( Cho Jin-Ho ) - Chungbuk National University Division of Food and Animal Sciences
 ( Song Min-Ho ) - Chungnam National University Division of Animal and Dairy Science
 ( Lee Ju-Hoon ) - Kyung Hee University Department of Food Science and Biotechnology
 ( Isaacson Richard E. ) - University of Minnesota Department of Veterinary and Biomedical Sciences
송근호 ( Song Kun-Ho ) - Chungnam National University College of Veterinary Medicine
 ( Kim Hyeun-Bum ) - Dankook University Department of Animal Resources Science

Abstract


Canine parvoviral enteritis (PVE) is an important intestinal disease of the puppies; however, the potential impact of the canine parvovirus (CPV) on the gut microbiota has not been investigated. Therefore, the aim of this study was to evaluate the gut microbial shifts in puppies naturally infected with CPV. Fecal samples were collected from healthy dogs and those diagnosed with PVE at 4, 6, 8, and 12 weeks of age. The distal gut microbiota of dogs was characterized using Illumina MiSeq sequencing of the bacterial 16S rRNA genes. The sequence data were analyzed using QIIME with an Operational Taxonomic Unit definition at a similarity cutoff of 97%. Our results showed that the CPV was associated with significant microbial dysbiosis of the intestinal microbiota. Alpha diversity and species richness and evenness in dogs with PVE decreased compared to those of healthy dogs. At the phylum level, the proportion of Proteobacteria was significantly enriched in dogs with PVE while Bacteroidetes was significantly more abundant in healthy dogs (p < 0.05). In dogs with PVE, Enterobacteriaceae was the most abundant bacterial family accounting for 36.44% of the total bacterial population compared to only 0.21% in healthy puppies. The two most abundant genera in healthy dogs were Prevotella and Lactobacillus and their abundance was significantly higher compared to that of dogs with PVE (p < 0.05). These observations suggest that disturbances of gut microbial communities were associated with PVE in young dogs. Evaluation of the roles of these bacterial groups in the pathophysiology of PVE warrants further studies.

키워드

Gut microbiota; canine parvovirus; dogs; 16S rRNA
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