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Ralstonia pickettii enhance the DDT biodegradation by Pleurotus eryngii

Journal of Microbiology and Biotechnology 2019년 29권 9호 p.1424 ~ 1433
 ( Purnomo Adi Setyo ) - Institut Teknologi Sepuluh Nopember Faculty of Science Department of Chemistry

 ( Maulianawati Diana ) - Borneo Tarakan University Faculty of Fisheries and Marine Science Department of Aquaculture
 ( Kamei Ichiro ) - University of Miyazaki Faculty of Agriculture Department of Forest and Environmental Science


DDT is a hydrophobic organic pollutant, which can be bio-accumulated in nature and have adverse consequences on the physical condition of humans and animals. This study investigated the relationship between the white-rot fungus Pleurotus eryngii and biosurfactantproducing bacterium Ralstonia pickettii associated with the degradation of DDT. The effects of R. pickettii on fungal development were examined using in vitro confrontation assay on a potato dextrose agar (PDA) medium. R. pickettii culture was added to the P. eryngii culture at 1, 3, 5, 7, and 10 ml (1 ml ? 1.44 × 1013 CFU). After 7 d incubation, about 43% of the initial DDT (12.5 μM) was degraded by the P. eryngii culture only. The augmentation of 7 ml of R. pickettii culture revealed a more highly optimized synergism with DDT degradation being approximately 78% and the ratio of optimization 1.06. According to the confrontational assay, R. pickettii promoted the growth of P. eryngii towards the bacterial colony, with no direct contact between the bacterial cells and mycelium (0.71 cm/day). DDD (1,1-dichloro-2,2-bis(4- chlorophenyl) ethane), DDE (1,1-dichloro-2,2-bis(4-chlorophenyl) ethylene), and DDMU (1- chloro-2,2-bis(4-chlorophenyl) ethylene) were identified as metabolic products, indicating that the R. pickettii could enhance the DDT biodegradation by P. eryngii.


Biodegradation; DDT; bacteria; white-rot fungi; Pleurotus eryngii; Ralstonia pickettii
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