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One-Step Engineering of a Stable, Selectable Marker-Free Autoluminescent Acinetobacter baumannii for Rapid Continuous Assessment of Drug Activity

Journal of Microbiology and Biotechnology 2019년 29권 9호 p.1488 ~ 1493
 ( Jiang Huofeng ) - University of Science and Technology of China School of Life Sciences

 ( Gao Yamin ) - Chinese Academy of Sciences Guangzhou Institutes of Biomedicine and Health State Key Laboratory of Respiratory Disease
 ( Zeng Sheng ) - Chinese Academy of Sciences Guangzhou Institutes of Biomedicine and Health State Key Laboratory of Respiratory Disease
 ( Wang Shuai ) - Chinese Academy of Sciences Guangzhou Institutes of Biomedicine and Health State Key Laboratory of Respiratory Disease
 ( Cao Zhizhong ) - Guangzhou Chest Hospital Department of Clinical Laboratory
 ( Tan Yaoju ) - Guangzhou Chest Hospital Department of Clinical Laboratory
 ( Yin Huancai ) - Chinese Academy of Sciences Suzhou Institute of Biomedical Engineering and Technology
 ( Liu Jianxiong ) - Guangzhou Chest Hospital Department of Clinical Laboratory
 ( Zhang Tianyu ) - Chinese Academy of Sciences Guangzhou Institutes of Biomedicine and Health State Key Laboratory of Respiratory Disease

Abstract


The rising cases of multidrug-resistant Acinetobacter baumannii (Ab) and the lack of effective drugs call for quick attention. Here, based on a Tn7 transposon and Xer/dif system, we constructed a stable, selectable marker-free autoluminescent Ab capable of producing visible light without extra substrates. Utilization of this autoluminescent reporter strain has the potential to reduce the time, effort and costs required for the evaluation of activities of anti-Ab drug candidates in vitro.

키워드

Autoluminescent; marker-free; Acinetobacter baumannii; Tn7; Xer/dif recombination system
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