[닫기]
잠시만 기다려 주세요. 로딩중입니다.

Establishment and Application of Polymerase Spiral Reaction Amplification for Salmonella Detection in Food

Journal of Microbiology and Biotechnology 2019년 29권 10호 p.1543 ~ 1552
 ( Xu Wenli ) - Northeastern University College of Life and Health Sciences

 ( Gao Jun ) - Northeastern University College of Life and Health Sciences
 ( Zheng Haoyue ) - Northeastern University College of Life and Health Sciences
 ( Yuan Chaowen ) - Northeastern University College of Life and Health Sciences
 ( Hou Jinlong ) - Northeastern University College of Life and Health Sciences
 ( Zhang Liguo ) - Center for Animal Disease Emergency of Liaoning Province
 ( Wang Guoqing ) - Northeastern University College of Life and Health Sciences

Abstract


Salmonella is a common zoonotic and foodborne pathogen that causes high morbidity and mortality in developing countries. In this study, we established and validated a polymerase spiral reaction (PSR) assay which targeted the conserved invasion gene (invA) of Salmonella by SYBR Green I indicator methods. Subsequently, assays for determination of the optimal conditions for optimal specificity and sensitivity of PSR were performed. We performed comprehensive evaluations using loop-mediated isothermal amplification (LAMP) and realtime PCR. A total number of 532 samples of daily food were analyzed by PSR. Twenty-seven bacterial strains were tested in the specificity assay, from which positive results were obtained only for 14-Salmonella strains. However, none of the 13 non-Salmonella strains was amplified. Similarly with LAMP and real-time PCR, the detection limit of the PSR assay was 50 CFU/ml. The PSR method was also successfully applied to evaluate the contamination with Salmonella in 532 samples of daily food, corroborating traditional culture method data. The novel PSR method is simple, sensitive, and rapid and provides new insights into the prevention and detection of foodborne diseases.

키워드

Salmonella; invasion gene A; isothermal amplification; rapid detection, food samples
원문 및 링크아웃 정보
등재저널 정보