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A Gene Cluster for the Biosynthesis of Dibenzodioxocinons in the Endophyte Pestalotiopsis microspora, a Taxol Producer

Journal of Microbiology and Biotechnology 2019년 29권 10호 p.1570 ~ 1579
 ( Liu Yanjie ) - Beijing Normal University College of Life Sciences Institute of Biochemistry and Biotechnology

 ( Chen Longfei ) - Zhejiang Medicine Co. Ltd.
 ( Xie Qiaohong ) - Beijing Normal University College of Life Sciences Institute of Biochemistry and Biotechnology
 ( Yu Xi ) - Nankai University College of Life Sciences Department of Microbiology
 ( Duan Anqing ) - Beijing Normal University College of Life Sciences Institute of Biochemistry and Biotechnology
 ( Lin Yamin ) - Beijing Normal University College of Life Sciences Institute of Biochemistry and Biotechnology
 ( Xiang Biyun ) - Beijing Normal University College of Life Sciences Institute of Biochemistry and Biotechnology
 ( Hao Xiaoran ) - Beijing Normal University College of Life Sciences Institute of Biochemistry and Biotechnology
 ( Chen Wanwan ) - Beijing Normal University College of Life Sciences Institute of Biochemistry and Biotechnology
 ( Zhu Xudong ) - Beijing Normal University College of Life Sciences Institute of Biochemistry and Biotechnology

Abstract


The fungal products dibenzodioxocinones promise a novel class of inhibitors against cholesterol ester transfer protein (CEPT). Knowledge as to their biosynthesis is scarce. In this report, we characterized four more dibenzodioxocinones, which along with a previously described member pestalotiollide B, delimit the dominant spectrum of secondary metabolites in P. microspora. Through mRNA-seq profiling in gα1Δ, a process that halts the production of the dibenzodioxocinones, a gene cluster harboring 21 genes including a polyketide synthase, designated as pks8, was defined. Disruption of genes in the cluster led to loss of the compounds, concluding the anticipated role in the biosynthesis of the chemicals. The biosynthetic route to dibenzodioxocinones was temporarily speculated. This study reveals the genetic basis underlying the biosynthesis of dibenzodioxocinone in fungi, and may facilitate the practice for yield improvement in the drug development arena.

키워드

Dibenzodioxocinones; CEPT inhibitor; Pestalotiollide B; polyketide synthase; Pestalotiosis microspora NK17
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