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Production of Bio-Based Isoprene by the Mevalonate Pathway Cassette in Ralstonia eutropha

Journal of Microbiology and Biotechnology 2019년 29권 10호 p.1656 ~ 1664
 ( Lee Hyeok-Won ) - Korea Research Institute of Bioscience and Biotechnology Biotechnology Process Engineering Center

 ( Park Jung-Ho ) - Korea Research Institute of Bioscience and Biotechnology Bio-Evaluation Center
 ( Lee Hee-Seok ) - Korea Research Institute of Bioscience and Biotechnology Biotechnology Process Engineering Center
 ( Choi Won-Ho ) - Korea Research Institute of Bioscience and Biotechnology Bio-Evaluation Center
 ( Seo Sung-Hwa ) - Korea Research Institute of Bioscience and Biotechnology Biotechnology Process Engineering Center
 ( Anggraini Irika Devi ) - Korea Research Institute of Bioscience and Biotechnology Biotechnology Process Engineering Center
 ( Choi Eui-Sung ) - Korea Research Institute of Bioscience and Biotechnology Biotechnology Process Engineering Center
 ( Lee Hong-Weon ) - Korea Research Institute of Bioscience and Biotechnology Biotechnology Process Engineering Center

Abstract


Isoprene has the potential to replace some petroleum-based chemicals and can be produced through biological systems using renewable carbon sources. Ralstonia eutropha can produce value-added compounds, including intracellular polyhydroxyalkanoate (PHA) through fatty acid and lipid metabolism. In the present study, we engineered strains of R. eutropha H16 and examined the strains for isoprene production. We optimized codons of all the genes involved in isoprene synthesis by the mevalonate pathway and manipulated the promoter regions using pLac and pJ5 elements. Our results showed that isoprene productivity was higher using the J5 promoter (1.9 ± 0.24 μg/l) than when using the lac promoter (1.5 ± 0.2 μg/l). Additionally, the use of three J5 promoters was more efficient (3.8 ± 0.18 μg/l) for isoprene production than a one-promoter system, and could be scaled up to a 5-L batch-cultivation from a T-flask culture. Although the isoprene yield obtained in our study was insufficient to meet industrial demands, our study, for the first time, shows that R. eutropha can be modified for efficient isoprene production and lays the foundation for further optimization of the fermentation process.

키워드

Ralstonia eutropha; isoprene production; promoter; fermentation; MVA pathway
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