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Pseudomonas syringae pv. tomato DC3000 Improves Escherichia coli O157:H7 Survival in Tomato Plants

Journal of Microbiology and Biotechnology 2019년 29권 12호 p.1975 ~ 1981
 ( Namgung Min ) - Kangwon National University Division of Bioresource Sciences

 ( Lim Yeon-Jeong ) - Kangwon National University Division of Bioresource Sciences
 ( Kang Min-Kyu ) - Kangwon National University Division of Bioresource Sciences
오창식 ( Oh Chang-Sik ) - Kyung Hee University Department of Horticultural Biotechnology
박덕환 ( Park Duck-Hwan ) - Kangwon National University Division of Bioresource Sciences

Abstract


Recently, outbreaks of food-borne diseases linked to fresh produce have been an emerging public health concerns worldwide. Previous research has shown that when human pathogens co-exist with plant pathogens, they have improved growth and survival rates. In this study, we have assessed whether Escherichia coli O157:H7 benefits in the existence of a phytopathogenic bacterium and the underlying mechanisms were further investigated. When Pseudomonas syringae pv. tomato DC3000 (Pst DC3000) and E. coli O157:H7 were co-inoculated by either dipping or infiltration methods, the populations of E. coli O157:H7 increased; however, no effect was observed when type three secretion system (T3SS) mutants were used instead, suggesting that E. coli O157:H7 benefits from the presence of Pst DC3000. In addition, this study confirmed that the E. coli O157:H7 populations increased when they occupied the tomato leaf intercellular space; this colonization of the interior of the leaves was possible due to the suppression of the PAMP triggered immunity (PTI) by Pst DC3000, in particular with the AvrPto effector. In conclusion, our data supports a plausible model that E. coli O157:H7 benefits from the presence of Pst DC3000 via AvrPto suppression of the PTI resistance.

키워드

AvrPto; E. coli O157:H7; effector; food-borne disease; Pseudomonas syringae pv. tomato DC3000; tomato
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