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Improving Pneumovirus Isolation Using a Centrifugation and AZD1480 Combined Method

Journal of Microbiology and Biotechnology 2019년 29권 12호 p.2006 ~ 2013
이한샘 ( Lee Han-Saem ) - Korea Centers for Diseases Control and Prevention National Institute of Health Center for Infectious Diseases Research

 ( Woo Hye-Min ) - Korea Centers for Diseases Control and Prevention National Institute of Health Center for Infectious Diseases Research
김기순 ( Kim Ki-Soon ) - Korea Centers for Diseases Control and Prevention National Institute of Health Center for Infectious Diseases Research
박세희 ( Park Se-Hee ) - Korea University College of Medicine Department of Microbiology
박만성 ( Park Man-Seong ) - Korea University College of Medicine Department of Microbiology
김성순 ( Kim Sung-Soon ) - Korea Centers for Diseases Control and Prevention National Institute of Health Center for Infectious Diseases Research
김유진 ( Kim You-Jin ) - Korea Centers for Diseases Control and Prevention National Institute of Health Center for Infectious Diseases Research

Abstract


The isolation of respiratory viruses, especially from clinical specimens, often shows poor efficiency with classical cell culture methods. The lack of suitable methods to generate virus particles inhibits the development of diagnostic assays, treatments, and vaccines. We compared three inoculation methods, classical cell culture, the addition of a JAK2 inhibitor AZD1480, and centrifugation-enhanced inoculation (CEI), to replicate human respiratory syncytial virus (HRSV) and human metapneumovirus (HMPV). In addition, a combined method using AZD1480 treatment and CEI was used on throat swabs to verify that this method could increase virus isolation efficiency from human clinical specimens. Both CEI and AZD1480 treatment increased HRSV and HMPV genome replication. Also, the combined method using CEI and AZD1480 treatment enhanced virus proliferation synergistically. The combined method is particularly suited for the isolation of interferon-sensitive or slowly growing viruses from human clinical specimens.

키워드

Virus isolation; centrifugation; AZD1480; human respiratory syncytial virus; human metapneumovirus
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