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Antioxidant and Anti-Obesity Activities of Polygonum cuspidatum Extract through Alleviation of Lipid Accumulation on 3T3-L1 Adipocytes

Journal of Microbiology and Biotechnology 2020년 30권 1호 p.21 ~ 30
 ( Choi Da-Hye ) - Chuncheon Bio-industry Foundation Department of Research and Development

 ( Han Joon-Hee ) - Chuncheon Bio-industry Foundation Department of Research and Development
 ( Yu Keun-Hyung ) - Chuncheon Bio-industry Foundation Department of Research and Development
 ( Hong Min ) - Chuncheon Bio-industry Foundation Department of Research and Development
 ( Lee Sun-Yeop ) - Chuncheon Bio-industry Foundation Department of Research and Development
 ( Park Ka-Hee ) - Chuncheon Bio-industry Foundation Department of Research and Development
이수웅 ( Lee Soo-Ung ) - Chuncheon Bio-industry Foundation Department of Research and Development
권태형 ( Kwon Tae-Hyung ) - Chuncheon Bio-industry Foundation Department of Research and Development

Abstract


Natural products are widely used due to their various biological activities which include antiinflammatory, antioxidant, and anti-obesity effects. In this study, we determined the antioxidative and anti-obesity effects of Polygonum cuspidatum 50% ethanol extract (PEE). The antioxidative effect of PEE was evaluated using its radical scavenging activity, total phenolic content, and reducing power. The anti-obesity effect of PEE was investigated using 3T3-L1 adipocytes. The antioxidative activity of PEE was progressively increased in various concentrations, mainly due to the presence of phenolic compounds. PEE also alleviated lipid accumulation on 3T3-L1 adipocytes and downregulated the mRNA and protein production of adipogenesis-related (SREBP-1c, PPARγ, C/EBPα) and lipogenesis-related (aP2, FAS, ACC) markers. Furthermore, we found that the inhibitory effect on lipid accumulation via PEE was caused by the alleviation of NF-κB, p38 MAPK, ERK1/2, and JNK at the protein level. Taken together, our results imply that PEE is a potential antioxidant that can prevent obesityassociated disorders.

키워드

3T3L1 cells; lipogenesis; adipogenesis; antioxidant; polygonum cuspidatum
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