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Trehalose Protects the Probiotic Yeast Saccharomyces boulardii against Oxidative Stress-Induced Cell Death

Journal of Microbiology and Biotechnology 2020년 30권 1호 p.54 ~ 61
 ( Moon Ji-Eun ) - korea university Department of Food and Biotechnology

 ( Heo Wan ) - Korea University Institute of Natural Sciences
이상훈 ( Lee Sang-Hoon ) - korea university Department of Food and Biotechnology
이석희 ( Lee Suk-Hee ) - Kyungpook National University School of Medicine Department of Molecular Medicine
이홍구 ( Lee Hong-Gu ) - Konkuk University Department of Animal Science and Technology
이진협 ( Lee Jin-Hyup ) - korea university Department of Food and Biotechnology
김영준 ( Kim Young-Jun ) - korea university Department of Food and Biotechnology

Abstract


Saccharomyces boulardii is the only probiotic yeast with US Food and Drug Administration approval. It is routinely used to prevent or treat acute diarrhea and other gastrointestinal disorders, including the antibiotic-associated diarrhea caused by Clostridium difficile infections. The formation of reactive oxygen species (ROS), specifically H2O2 during normal aerobic metabolism, contributes to programmed cell death and represents a risk to the viability of the probiotic microbe. Moreover, a loss of viability reduces the efficacy of the probiotic treatment. Therefore, inhibiting the accumulation of ROS in the oxidant environment could improve the viability of the probiotic yeast and lead to more efficacious treatment. Here, we provide evidence that supplementation with a non-reducing disaccharide, namely trehalose, enhanced the viability of S. boulardii exposed to an oxidative environment by preventing metacaspase YCA1-mediated programmed cell death through inhibition of intracellular ROS production. Our results suggest that supplementation with S. boulardii together with trehalose could increase the viability of the organism, and thus improve its effectiveness as a probiotic and as a treatment for acute diarrhea and other gastrointestinal disorders.

키워드

Trehalose; probiotics; reactive oxygen species; programmed cell death
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