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sRNA EsrE Is Transcriptionally Regulated by the Ferric Uptake Regulator Fur in Escherichia coli

Journal of Microbiology and Biotechnology 2020년 30권 1호 p.127 ~ 135
 ( Hou Bingbing ) - East China University of Science and Technology State Key Laboratory of Bioreactor Engineering

 ( Yang Xichen ) - East China University of Science and Technology State Key Laboratory of Bioreactor Engineering
 ( Xia Hui ) - East China University of Science and Technology State Key Laboratory of Bioreactor Engineering
 ( Wu Haizhen ) - East China University of Science and Technology State Key Laboratory of Bioreactor Engineering
 ( Ye Jiang ) - East China University of Science and Technology State Key Laboratory of Bioreactor Engineering
 ( Zhang Huizhan ) - East China University of Science and Technology State Key Laboratory of Bioreactor Engineering

Abstract


Small RNAs (sRNAs) are widespread and play major roles in regulation circuits in bacteria. Previously, we have demonstrated that transcription of esrE is under the control of its own promoter. However, the regulatory elements involved in EsrE sRNA expression are still unknown. In this study, we found that different cis-regulatory elements exist in the promoter region of esrE. We then screened and analyzed seven potential corresponding trans-regulatory elements by using pull-down assays based on DNA affinity chromatography. Among these candidate regulators, we investigated the relationship between the ferric uptake regulator (Fur) and the EsrE sRNA. Electrophoresis mobility shift assays (EMSAs) and β-galactosidase activity assays demonstrated that Fur can bind to the promoter region of esrE, and positively regulate EsrE sRNA expression in the presence of Fe2+.

키워드

Small RNA; EsrE; DNA affinity chromatography; Fur; transcriptional regulation
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