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Enhanced Lipid Production of Chlorella sp. HS2 Using Serial Optimization and Heat Shock

Journal of Microbiology and Biotechnology 2020년 30권 1호 p.136 ~ 145
 ( Kim Hee-Su ) - Korea Advanced Institute of Science and Technology Department of Chemical and Biomolecular Engineering

 ( Kim Min-Sik ) - Korea Advanced Institute of Science and Technology Department of Chemical and Biomolecular Engineering
박원근 ( Park Won-Kun ) - Sangmyung University Department of Chemistry and Energy Engineering
장용근 ( Chang Yong-Keun ) - Korea Advanced Institute of Science and Technology Department of Chemical and Biomolecular Engineering

Abstract


Chlorella sp. HS2, which previously showed excellent performance in phototrophic cultivation and has tolerance for wide ranges of salinity, pH, and temperature, was cultivated heterotrophically. However, this conventional medium has been newly optimized based on a composition analysis using elemental analysis and ICP-OES. In addition, in order to maintain a favorable dissolved oxygen level, stepwise elevation of revolutions per minute was adopted. These optimizations led to 40 and 13% increases in the biomass and lipid productivity, respectively (7.0 and 2.25 g l-1d-1 each). To increase the lipid content even further, 12 h heat shock at 50oC was applied and this enhanced the biomass and lipid productivity up to 4 and 17% respectively (7.3 and 2.64 g l-1d-1, each) relative to the optimized conditions above, and the values were 17 and 14% higher than ordinary lipid-accumulating N-limitation (6.2 and 2.31 g l-1d-1). On this basis, heat shock was successfully adopted in novel Chlorella sp. HS2 cultivation as a lipid inducer for the first time. Considering its fast and cost-effective characteristics, heat shock will enhance the overall microalgal biofuel production process.

키워드

Chlorella sp. HS2; fed-batch cultivation; elemental analysis; heat shock stress; lipid productivity
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