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Functional Expression and Characterization of Acetyl Xylan Esterases CE Family 7 from Lactobacillus antri and Bacillus halodurans

Journal of Microbiology and Biotechnology 2020년 30권 2호 p.155 ~ 162
김민정 ( Kim Min-Jeong ) - Chungbuk National University Division of Animal, Horticultural, and Food Sciences

장명운 ( Jang Myoung-Uoon ) - Chungbuk National University Division of Animal, Horticultural and Food Sciences
남경화 ( Nam Gyeong-Hwa ) - Chungbuk National University Division of Animal, Horticultural and Food Sciences
 ( Shin Hee-Ji ) - Chungbuk National University Division of Animal, Horticultural and Food Sciences
 ( Song Jeong-Rok ) - Chungbuk National University Division of Animal, Horticultural and Food Sciences
김태집 ( Kim Tae-Jip ) - Chungbuk National University Division of Animal, Horticultural and Food Sciences

Abstract


Acetyl xylan esterase (AXE; E.C. 3.1.1.72) is one of the accessory enzymes for xylan degradation, which can remove the terminal acetate residues from xylan polymers. In this study, two genes encoding putative AXEs (LaAXE and BhAXE) were cloned from Lactobacillus antri DSM 16041 and Bacillus halodurans C-125, and constitutively expressed in Escherichia coli. They possess considerable activities towards various substrates such as p-nitrophenyl acetate, 4-methylumbelliferyl acetate, glucose pentaacetate, and 7-amino cephalosporanic acid. LaAXE and BhAXE showed the highest activities at pH 7.0 and 8.0 at 50oC, respectively. These enzymes are AXE members of carbohydrate esterase (CE) family 7 with the cephalosporine-C deacetylase activity for the production of antibiotics precursors. The simultaneous treatment of LaAXE with Thermotoga neapolitana β-xylanase showed 1.44-fold higher synergistic degradation of beechwood xylan than the single treatment of xylanase, whereas BhAXE showed no significant synergism. It was suggested that LaAXE can deacetylate beechwood xylan and enhance the successive accessibility of xylanase towards the resulting substrates. The novel LaAXE originated from a lactic acid bacterium will be utilized for the enzymatic production of D-xylose and xylooligosaccharides.

키워드

Acetyl xylan esterases; carbohydrate esterase (CE) family 7; synergistic xylan degradation; Lactobacillus antri; Bacillus halodurans
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